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Specifications
Specifications
| Preparation Method | This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. |
| Quantity | 0.5 mg |
| Content And Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Target | Jurkat |
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