missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Microplates
  4. Microplate Strips
  5. Thermo Scientific™ Immuno Breakable Modules in White and Black

Thermo Scientific™ Immuno Breakable Modules in White and Black
BIOPHARMA

Product Code. 10063743
Change view
Click to view available options
Surface Treatment:
MaxiSorp
PolySorp
Universal Binding
Color:
Black
White
Unit Size:
Pack of 50
Pack of 60
5 product options available for selection
Product selection table with 5 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Surface Treatment Color unitSize
10063743 MaxiSorp Black Pack of 60
10521834 PolySorp Black Pack of 60
10435205 PolySorp White Pack of 60
10436473 MaxiSorp White Pack of 60
10089174 Universal Binding Black Pack of 50
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
5 options
This item is not returnable. View return policy
Product Code. 10063743 Supplier Thermo Scientific™ Supplier No. 446471
Only null left
Add to basket
571.90 EUR valid until 2026-06-30
BEST PRICE on Promo! Use promo code "28170" to get your promotional price.
Add to basket
This item is not returnable. View return policy

Description

Keep wells in place with these breakable modules in white and black, ensuring fluorescence and luminescence assays are easy to handle.

Keep wells securely in place with Thermo Scientific™ Breakable Modules in White and Black, ensuring fluorescence and luminescence assays are easy to handle.

Features:

  • High-quality polystyrene wells
  • Choice of two passive surfaces to enable assay optimization
  • C8 strips provide flexibility with sample size and optimal washing performance
  • Easy to handle; single well held as easily as strips
  • Compatible with common instrumentation

Choice of Surface:

  • Thermo Scientific™ PolySorp™ and Universal Binding (UB) are for adsorption of hydrophobic molecules.
  • Thermo Scientific™ MaxiSorp™ is hydrophilic and ideal for antibody sandwich assays.

White:

  • Opaque and reflective surface
  • Low cross talk and minimal background

Black:

  • Opaque and light-absorbing surface
  • Minimum cross talk and back-scattered light

Note:

Availability may vary by country.

TRUSTED_SUSTAINABILITY

Specifications

Breakapart Breakable Strips
Binding Property Hydrophilic
Certified Yes
Format Strip Plate
Detection Method Fluorescence
Array 8 x 12
Packaging 60 Cases
Binding Type Adsorption
Color Black
Product Type Microplate
Material Polystyrene
Volume (Metric) Well 350 μL
No. of Wells 96
Volume (Metric) Working 250 μL
Surface Treatment MaxiSorp
Well Shape Round, C Bottom
For Use With (Application) Fluorescence
No. per Case 60
No. per Pack 10
Plate Blocking None
Target Molecule Antibodies (Glycoproteins)
Show More Show Less

Frequently Asked Questions (FAQs)

What length of peptide is ideal for binding to the Thermo Scientific MaxiSorp surface? What are the detection limitations?

We have tested and found that a 3 amino acid peptide (Pro, Leu, Gly) cannot be detected when passively adsorbed on the MaxiSorp surface. However, this peptide can be detected when covalently immobilized using CovaLink NH Modules and CovaLink NH2 Modules and Plates. Using covalent immobilization of small peptide residues, you can typically obtain a better orientation of the molecule and reduced problems with antibody recognition of the peptide due to masking of the epitope. We have discovered that a 7 amino acid peptide from the MHC Class II antigen can be detected when adsorbed on the MaxiSorp surface. We state that the detection limitation using the MaxiSorp surface is between 3 and 7 amino acid residues.
One additional note is that detection is contingent upon the orientation of the peptide when immobilized. If the active site is inactivated or hidden at the site facing the solid phase, no detection signal is observed.

Can I bind either single- or double-stranded DNA to the Thermo Scientific MaxiSorp surface?

Single-stranded DNA can be adsorbed to our MaxiSorp surface using approximately 10 µg ssDNA per mL PBS, pH 8.2, although the stability is uncertain. Based on our experience, ssDNA immobilized on the MaxiSorp surface is so loosely bound that it can be removed by stringent washing.
Double-stranded DNA will not bind to the MaxiSorp surface. DNA, however, can be covalently bound to Nunc NucleoLink Strips.
For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

What is the difference between certified and non-certified MaxiSorp plates and modules?

Both of these surfaces are identical. The only difference between them is that for the certified plates, a representative sample from each manufacturing lot undergoes a Binding Capacity test. This test is an ELISA-like assay used in our quality control laboratories to ensure binding capabilities.
How can I reduce high background readings and non-specific binding, when performing ELISA using Nunc-Immuno MaxiSorp Plates?

Assay sensitivity depends strongly on efficient removal of non-specific reacting molecules. High background readings and coating instability can be minimized by addition of a blocking step after the first coating. The excess surface is then occupied by indifferent molecules.
We recommend washing three times after each coating step by using a solution of 0.15 M phosphate buffer (pH 7.2) with 0.2 M NaCl and 0.05% Tween 20.
For blocking, we recommend using 0.5% BSA, 1% casein or 1% gelatin in 0.15 M phosphate buffer (pH 8.2) or carbonate buffer (pH 9.6).

Which surfaces do you recommend for RIA (radioimmunoassay)?

The choice of plates or modules will depend on the type of RIA you are planning to perform and specific assay conditions. Nunc provides two different surfaces for immunoassays, PolySorp and MaxiSorp.
- MaxiSorp surface is highly charged and should be used if the assay requires quantitative measurement of proteins (antibodies) or molecules with polar groups.
- PolySorp surface should be used if the assay consists of less polar molecules with hydrophobic characteristics.
Which 96-well Immuno plates or modules do you recommend for various applications?

The following list offers a brief description of the features of Nunc products and their specific applications:
- Nunc-Immuno Plates, MaxiSorp surface - These plates are designed for solid phase immuno assays and have a polystyrene surface with high affinity for polar groups and hydrophilic molecules. These 96-well plates are available with flat (F), round (U), or (C) bottom-well designs.
- Nunc-Immuno Plates, PolySorp surface - These plates have a polystyrene surface which adsorps less polar molecules compared to the MaxiSorp surface and has a high affinity for hydrophobic groups. These 96-well plates are available with flat (F), round (U), or (C) bottom-well designs.
- Nunc-Immuno Modules - These modules are designed for solid-phase immuno assays. The modules are available in 8-, 12- or 16-well formats with (F), (U), and (C) bottom-well and 8-well BreakApart with (C) bottom-well designs. These different formats allow one to choose the style which is appropriate for their assay design.
BreakApart Modules and LockWell Modules can be used for radioimmunoassays.
- Nunc StarWell Immuno Breakable Modules - The 8-well modules feature eight fins on the inner wall of the (C) bottom wells. This design increases the surface area by 50%. The increase in surface area allows more molecules to be immobilized, increasing assay signal. The fin configuration provides shorter diffusion distance to the surface, reducing incubation times.
- LockWell Immuno Breakable Modules - Each plate consists of 1 x 8 breakable strips. These strips are assembled in a designed frame which locks each well into place by a spring lock. This spring lock design orientates each well at the same horizontal level allowing uniform washing and reading. The LockWell Modules are available with round (U), (C) or StarWell bottom-well designs.
- Plates and Modules with (CovaLink) Covalent Binding Surfaces: These plates can be used to bind proteins, peptides, DNA or carbohydrates. Covalent linkage occurs via secondary amine group. This allows a specific orientation and provides improved stability compared to passive adsorption due to chemical binding between molecules. Binding of molecules on PolySorp and MaxiSorp surfaces is obtained by passive adsorption. CovaLink is a surface grafted with secondary amino groups (<NH) which serves as a bridge for covalent coupling. The covalent binding can immobilize small molecules such as biotin or peptides which may otherwise bind weakly by physical adsorption and facilitate the recognition by detection molecule.
- Nunc FluoroNunc/LumiNunc 96-Well Plates: These plates are optimized for IFMA (Immunofluorometric Assays) or FIA (Fluorometric Immuno Assays). The transparent polystyrene plates give a low background fluorescence and are optimal where a read-through system is used. The white plates/modules provide maximum reflection of fluorescence signal while maintaining low background. The white plates/modules are often used for epifluorescence reading. Black modules reduce background fluorescence and minimize back-scatter light which is often encountered in epifluorescence.

What is the maximum binding capacity for proteins on the Thermo Scientific MaxiSorp surface and PolySorp surface?

Molecules bind to our PolySorp and MaxiSorp surfaces through passive adsorption.
Using IgG as a reference molecule and knowing that it is a globular molecule, theoretical calculations indicate that the maximum binding for proteins are:
- For the MaxiSorp surface, in monolayer: 650 ng/cm^2
- For the PolySorp surface: 220 ng/cm^2.
What is the difference between your Thermo Scientific PolySorp, MaxiSorp, and MiniSorp surfaces?

MaxiSorp, PolySorp and MiniSorp surfaces were developed for immunology assays:
- The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity for molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation. Thus, it offers very high sensitivity in double antibody "sandwich" tests.
- The PolySorp surface is more hydrophobic than the MaxiSorp surface. It has high affinity to molecules of a more hydrophobic character. This surface is particularly suited to non-protein antigens including virus antigens.
- The MiniSorp surface is a polyethylene surface with very low affinity to molecules of any type. This type of surface is ideal for the liquid phase immuno techniques.
- The MiniSorp surface is only available in a tube format, while the MaxiSorp and PolySorp surfaces are offered in both 96-well plate and module formats.
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.