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Cytiva Single Cell GenomiPhi™ DNA Amplification Kit
Tienda Cytiva Productos
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No. of Reactions:
25
100
Tamaño de la unidad:
100 reactions
25 reactions
Descripción
Includes
Single Cell GenomiPhi Reaction buffer 1 x 275µL, Single Cell GenomiPhi Enzyme mix 1 x 25µL, Single Cell GenomiPhi Amplification mix 1 x 25µL, Single Cell GenomiPhi DTT Solution 1 x 100µL, Single Cell GenomiPhi Lysis buffer 1 x 100µL and Single Cell GenomiPhi Neutralisation buffer 1 x 100µL
- For whole genome amplification of genomic DNA from as little as a single cell
- Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
- Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
- Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
- Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
- Reaction time: 2 to 4 hours
- Product average size: >10kb
For SNP arrays, Next Gen Sequencing, aCGH
Recommended StorageStore the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C
Especificaciones
Especificaciones
| Content And Storage | Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. |
| Description | Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference. |
| Final Product Type | illustra™ Single Cell GenomiPhi™ DNA Amplification Kit |
| For Use With (Application) | Genomic DNA Amplification |
| Includes | Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL |
| No. of Reactions | 25 |
| Product Type | illustra Single Cell GenomiPhi DNA Amplification Kit |
| Size | >10kb |
| Test Time | 2 to 4 hours |
Seguridad y manipulación
Palabra de advertencia
- Danger
- Skin corrosion/irritation Category 1
- Skin corrosion/irritation Category 1 B
- H314-Causes severe skin burns and eye damage.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : potassium hydroxide
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