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  5. Invitrogen™ IL-9 Human ELISA Kit

Invitrogen™ IL-9 Human ELISA Kit

Product Code. 15581597
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Quantity:
96 Tests
Unit Size:
96 tests
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Product Code. 15581597

Brand: Invitrogen™ BMS2081

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Description

Includes

  • Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-9
  • Biotin-Conjugate anti-human IL-9 monoclonal antibody
  • Streptavidin-HRP
  • Human IL-9 Standard lyophilized, 200pg/mL upon reconstitution
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  • Sample Diluent
  • Calibrator Diluent
  • Substrate Solution (tetramethyl-benzidine)
  • Stop Solution (1M Phosphoric acid)
  • Blue-Dye
  • Green-Dye
  • Red-Dye
  • Adhesive Films

For the quantitative detection of human IL-9.

The Human Interleukin-9 (Hu IL-9) ELISA quantitates Hu IL-9 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-9. Principle of the method The Human IL-9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P15248
Assay Range 3.1-200 pg/mL
Assay Sensitivity 0.5 pg/mL
Conjugate Biotin
Product Type ELISA Kit
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3578
Gene Symbol HP40,IL-9,P40
Interassay CV 4.8%
Intraassay CV 5.3%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Storage Requirements 2°C to 8°C
Synonym Interleukin 9, MEA, HP40, P40 cytokine
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 3 hr. 30 min.
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For Research Use Only

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