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Invitrogen™ Human IL-12 (p70) ELISA Kits

Product Code. 10465725
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Quantity:
1 Plate Kit
Unit Size:
1 set
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Product Code. 10465725

Brand: Invitrogen™ EHIL12

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Description

Measure levels of active human interleukin-12, p70 in human serum, plasma, urine and culture supernatant samples using 96-well plates.

Assay IL-12 p70 (active human interleukin-12, p70) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-12 (p70) ELISA Kits.

These human IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically active interleukin-12 (IL-12 p70) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Biologically active (p70) natural and recombinant human interleukin-12 (IL-12, p70)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, TNFα, IFNγ, GM-CSF, GROα or GROβ. Recombinant human IL-12 (p40) and mouse IL-12 (p40) do not cross-react or interfere with this assay

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 15.4 to 600pg/mL
Assay Sensitivity <3pg/mL
Description Human IL-12 (p70) ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Sample Type Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
Specificity No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, TNFa, IFNg, GM-CSF, GROa or GROß. Recombinant human IL-12 (p40) and mouse IL-12 (p40) do not cross-react or interfere with t
Sufficient For 96 microplate wells
Kit Contents 30X Wash Buffer, 50mL, Stop Solution, 13mL, Adhesive Plate Covers, 6 ea, Biotinylated Antibody Reagent, 8mL, HRP Dilution Buffer, 13mL, Anti-Human IL-12 Precoated 96-well Strip Plate, 1 ea, Standard Diluent, 14mL, Streptavidin-HRP Concentrate, 75 μL, TMB Substrate Solution, 13mL, Lyophilized Recombinant Human IL-12 Standard, 2 vials
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
Quantity 1 Plate Kit
Target Biologically active (p70) natural and recombinant human interleukin-12 (IL-12, p70)
Total Assay Time 3 hr.
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