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Invitrogen™ Human I-TAC ELISA Kits

Product Code. 12341873
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Quantity:
1 Plate Kit
Unit Size:
Each
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Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 12341873

Brand: Invitrogen™ EHITAC

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This item is not returnable. View return policy
Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Description

Measure levels of human interferon-inducible T-cell alpha chemoattractant in human serum, plasma and culture supernatant samples using 96-well plates.

Assay I-TAC (human interferon-inducible T-cell alpha chemoattractant) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Invitrgen™ Human I-TAC ELISA Kits.

These human I-TAC ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of I-TAC (also known as CXCL11 or IP9) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human I-TAC (human interferon-inducible T-cell alpha chemoattractant, also called CXCL11, IP9)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 41.0 to 4000pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human MIG/CXCL9, IP-10/CXCL10, IL-8, IFNα or mouse ITAC/CXCL11

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 41.0 to 4000pg/mL
Assay Sensitivity <5pg/mL
Description Human I-TAC ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Sample Type Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Specificity No significant cross-reaction with human MIG/CXCL9, IP-10/CXCL10, IL-8, IFNa or mouse ITAC/CXCL11
Sufficient For 96 microplate wells
Kit Contents Streptavidin-HRP Concentrate, 75 μL, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Standard Diluent, 28mL, 30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Human I-TAC Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 8mL, Lyophilized Recombinant Human I-TAC Standard, 2 vials, HRP Dilution Buffer, 14mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
Quantity 1 Plate Kit
Sample Volume 50 μL
Target Natural and recombinant human I-TAC (human interferon-inducible T-cell alpha chemoattractant, also called CXCL11, IP9)
Total Assay Time 3.5 hr.
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