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Description
KE00255 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human HO-1/HMOX1 ELISA kit is to be used to detect and quantify protein levels of endogenous human HO-1/HMOX1. The assay recognizes human HO-1/HMOX1. An antibody specific for human HO-1/HMOX1 has been pre-coated onto the microwells. The human HO-1/HMOX1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody for human HO-1/HMOX1 is added to detect the captured human HO-1/HMOX1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Specifications
Specifications
| Assay Sensitivity | 0.08 pg/mL |
| Assay | Sandwich |
| Sample Type | Cell Lysates, Plasma, Serum |
| Sufficient For | 96 Tests |
| For Use With (Application) | Sandwich ELISA |
| Quantity | 1 x 96 well plate |
| Target | Human |
Safety and Handling
Product Identifier
- Human HO-1/HMOX1 ELISA Kit, 96 RXN
- Warning
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
Product Title
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