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Proteintech™ Human Haptoglobin ELISA Kit

Product Code. 16851556 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. 16851556

Brand: Proteintech™ KE0014896T

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This item is not returnable. View return policy

Description

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous haptoglobin

  • Recognizes human haptoglobin
  • Haptoglobin (HP, zonulin) is a plasma glycoprotein belonging to peptidase S1 family
    • Acute-phase response protein, serum Hp is mainly synthesized by hepatocytes and distributed in circulation
    • Main physiological function of serum Hp is binding free haemoglobin released from red blood cell lysis and protecting tissues and vessels from oxidative damage
    • Level Hp useful biomarker for some diseases diagnosis (Hepatocellular carcinoma, pancreatic cancer, Type 2 diabetes mellitus, diabetes kidney disease)

Process:

  • Antibody specific for haptoglobin pre-coated onto microwells
  • Haptoglobin protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for human haptoglobin is added to detect captured human haptoglobin protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifications

Accession Number BC058031
Assay Range 62.5 to 4000 pg/mL
Assay Sensitivity 3 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 3240
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P00738
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Sicurezza e movimentazione

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Identificativo prodotto
  • 96TEST Human Haptoglobin ELISA Kit
 Avvertenza
  • Warning
 Categoria di pericolo
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Indicazioni di pericolo
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Consigli di prudenza
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Informazioni supplementari
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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