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Proteintech™ Human FKBPL ELISA Kit

Product Code. 16811456 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. 16811456

Brand: Proteintech™ KE0002396T

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This item is not returnable. View return policy

Description

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous FKBPL

  • Recognizes human FKBPL
  • FKBPL (DIR1, NG7, WISp39) similar to immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking
    • Thought to have potential role in induced radioresistance
    • Breast cancer cells stably overexpressing FKBPL became dependent on estrogen for growth
    • FKBPL is an estrogen-inducible gene that acts as a co-chaperone in ERa/Hsp90 molecular complexes
      • Levels may be prognostic indicator and determinant of response to endocrine therapy

Process:

  • Antibody specific for FKBPL pre-coated onto microwells
  • FKBPL protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for FKBPL is added to detect captured FKBPL protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, meaurable at 450 nm with correction wavelength set at 630 nm

Specifications

Accession Number NP_071393
Assay Range 31.25 to 2000 pg/mL
Assay Sensitivity 7 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 63943
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 3.5 hr.
UniProt ID Q9UIM3
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Safety and Handling

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Product Identifier
  • 96TEST Human APCS ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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