Proteintech Human FABP2 ELISA Kit

Description

KE00263 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FABP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human FABP2. An antibody specific for FABP2 has been pre-coated onto the microwells. The FABP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for FABP2 is added to detect the captured FABP2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.