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  6. Cytiva HisTrap HP, 100 Columns

Cytiva HisTrap HP, 100 Columns

Product Code. 11555674 Shop All Cytiva Products
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Quantity:
100 x 1 mL
100 x 5 mL
Bed Volume:
1 mL
5 mL
Unit Size:
Pack of 100
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Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 11555674

Brand: Cytiva 17524705

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This item is not returnable. View return policy
Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Beskrivning

Ensure reliable, high-yield capture of histidine-tagged proteins in IMAC. GE Healthcare HisTrap HP, 100 Columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification by immobilized metal ion affinity chromatography (IMAC).

HisTrap HP 1ml and 5ml columns are designed for one-step purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose High Performance to ensure reliable capture of target protein in repeated IMAC purifications. The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield. At the same time, it greatly expands the range of suitable operating conditions.
  • High binding capacity—at least 40mg/ml chromatography medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump or chromatography system, such as ÄKTA design
  • Can be used for purification of a range of tagged proteins
  • 100-column bulk pack for convenience and economy
HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be co-purified with the tagged target protein.For convenient scaling up of histidine-tagged protein purification, use 20 ml HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.
TRUSTED_SUSTAINABILITY

Specifikationer

Bed Volume 1 mL
Binding Capacity At least 40 mg histidine-tagged protein/mL medium, Protein binding capacity is protein-to-protein dependent.
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Material Polypropylene (PP)
Matrix Highly cross-linked agarose, 6%
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 34 μm
Quantity 100 x 1 mL
Type Pre-packed Column

Säkerhet och hantering

Signalord
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 Riskkategorier
  • Flammable liquid Category 3
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