missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

180.00€ - 9260.00€

Specifications

Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Content And Storage Store at 4°C
Form Liquid Suspension
Product Line HisPur™
Target His-Tagged Protein
View More Specs

Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
13414199
View Documents Promotion Details
Thermo Scientific™
25217
1 L
9260.00€
1 L
View alternative products
Save Up to 
»
Estimated Shipment: 09-04-2024
to see stock.
Add to basket
 
13484189
View Documents Promotion Details
Thermo Scientific™
25214
10 mL
180.00€
10mL
View alternative products
Save Up to 
»
In Stock
to see stock.
Add to basket
 
13404199
View Documents Promotion Details
Thermo Scientific™
25215
50 mL
738.00€
50mL
View alternative products
Save Up to 
»
In Stock
to see stock.
Add to basket
 
13494189
View Documents Promotion Details
Thermo Scientific™
25216
250 mL
2875.00€
250mL
View alternative products
Save Up to 
»
Estimated Shipment: 09-04-2024
to see stock.
Add to basket
 
Description

Description

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).


Additional Information and Online Ordering »

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

Specifications

Specifications

20 mg His-Tagged GFP Binding per mL Resin
Liquid Suspension
His-Tagged Protein
Divalent Nickel, Metal Chelate
Affinity, Agarose Resin
Store at 4°C
HisPur™
Agarose
Bottle
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.