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  6. Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose
  7. 13404199

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Brand:  Thermo Scientific™ 25215

Additional Details : CAS : 64-17-5 Weight : 0.05000kg Transport : UN number : 1993 Chem class : 3 Pack group : III


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Product Code. 13404199

  • 738.00€ / 50mL
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Description

Description

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).


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HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

Specifications

Specifications

20 mg His-Tagged GFP Binding per mL Resin
Liquid Suspension
50 mL
Agarose
Bottle
Store at 4°C
HisPur™
His-Tagged Protein
Divalent Nickel, Metal Chelate
Affinity, Agarose Resin
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Safety and Handling

Safety and Handling

Product Identifier
  • HisPur Ni-NTA Superflow Agarose, 50 mL.

Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Skin sensitiser Category 1
  • Respiratory sensitiser Category 1
  • Carcinogenicity Category 1
  • Reproductive toxicity Category 1B
  • Specific target organ toxicity after repeated exposure Category 2
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H350-May cause cancer.
  • H360D-May damage the unborn child.
  • H373-May cause damage to organs.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P370+P378-In case of fire: Use to extinguish.

Supplemental information
  • MIXTURE LIST-Contains : Ethyl alcohol, Nickel(II) sulfate hexahydrate(1:1:6)

SDS
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Documents

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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.