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Abcam Fluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue)
Description
MW 740.6 Da, Purity >98%. Fluo-5 AM is an analogue of Fluo-4 with reduced calcium binding affinity (Kd = 2.3 μM). Suitable for detecting intracellular calcium levels in the range 1 μM to 1 mM. Cells can be loaded with Fluo-5 AM by adding the dissolved indicator directly to cells in culture dishes.
Fluo-5 AM has excitation and emission wavelengths at 494 and 516 nm respectively. Compatible with excitation by argon-ion lasers at 488 nm. Fluorescence inceases by >100-fold upon binding calcium.
.The product is subject to the following: Abcam Restricted Use Statement
Specifications
Specifications
| Quantity | 10 x 50 μg |
| Regulatory Status | RUO |
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