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Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)
Optimize routine PCR with this highly thermostable DNA polymerase.
Brand: Thermo Scientific™ EP0403
Additional Details : Weight : 0.01000kg
Description
Optimize routine PCR with this highly thermostable DNA polymerase.
Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Thermostable -half life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
- Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Specifications
| 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO2, 2 x 1.25 mL 10X Taq Buffer with KCl 25 and 2 x 1.25 mLmM MgCl2 | |
| 100 Units | |
| Half life is more than 40 min. at 95°C |
| Taq DNA Polymerase (recombinant), 1 U/μL | |
| Standard PCR, DNA Sequencing |
For Research Use Only. Not for use in diagnostic procedures.