missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Mva1269I (BsmI) (10 U/µL)

The Mva1269I (BsmI) restriction enzyme recognizes GAATGC(1/-1)^ sites and cuts best at 37°C in R buffer (Isoschizomers: BsaMI, BsmI, PctI).

Brand:  Thermo Scientific™ ER0962

Additional Details : Weight : 1.51950kg

 View more versions of this product

Product Code. 10120790

  • 197.00€ / 1000 units

In Stock
to see stock.
Add to basket



Lambda DNA digested with Mva1269I (BsmI), 1% agarose, 46 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G A A T G C N▵...3'

3'...C T T A C▵G N ...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Mva1269I is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours


10 U/μL
Mva1269I (BsmI)
Restriction Enzyme
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer R
1000 U
Scarless Cloning


Special Offers

Special Offers