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Descripción
Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientitfic restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
The marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.
Highlights
- Sizing and approximate quantification of large DNA fragments
- Sharp bands
- Supplied with loading dye for sample DNA
Especificaciones
Especificaciones
| Gel Type | Agarose |
| Ready to Load | No |
| Gel Compatibility | Agarose Gels |
| Quantity | 5 x 50 μg |
| Product Type | DNA/Hind III Marker |
Frequently Asked Questions (FAQs)
Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
For Research Use Only. Not for use in diagnostic procedures.
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