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  5. Thermo Scientific™ GeneJET RNA Cleanup and Concentration Micro Kit

Thermo Scientific™ GeneJET RNA Cleanup and Concentration Micro Kit

Artikelnummer. 13213159 Shop alle Thermo Scientific produkter
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Quantity:
250 Preps
50 Preps
Pakningsstørrelse:
250 tests
50g
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Artikelnummer. 13213159 Leverandør Thermo Scientific™ Leverandørnr. K0842
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Beskrivelse

GeneJET RNA Cleanup and Concentration Micro Kit is designed for rapid and efficient concentration of prepurified RNA samples, as well as for RNA cleanup after DNase I treatment and other enzymatic reactions.

GeneJET RNA Cleanup and Concentration Micro Kit is designed for rapid and efficient concentration of prepurified RNA samples, as well as for RNA cleanup after DNase I treatment and other enzymatic reactions.

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Thermo Scientific GeneJET RNA Cleanup and Concentration Micro Kit is designed for rapid and efficient concentration of prepurified RNA samples, as well as for RNA cleanup after DNase I treatment and other enzymatic reactions.

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Highlights

  • Advanced column design—easy to open, load and handle.
  • Elute in 6 to 10 μL volume.
  • 85 to 100% recovery of a high integrity RNA.
  • Fast and simple—procedure takes less than 5 minutes.

Applications:

  • The purified RNA can be used in common downstream applications
  • RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis
TRUSTED_SUSTAINABILITY

Tekniske data

Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format Spin Column
Isolation Technology Spin Column
Purification Time 5 min.
Sample Type RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction
No. of Reactions 250 Preps
Product Line GeneJET
Quantity 250 Preps
Scale Micro
Target Total RNA
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Frequently Asked Questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
Is any GeneJET kit suitable for RNA purification from formalin-fixed paraffin-embedded FFPE tissues?

We cannot recommend GeneJET kits for RNA purification from FFPE tissues. In case of genomic DNA purififcation from formalin-fixed paraffin-embedded samples, we recommend GeneJET FFPE DNA Purification Kit.
Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.
At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.
Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

Sikkerhed og håndtering

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Produktidentifikator
  • GeneJET RNA Cleanup and Concentration Micro Kit
 Signalord
  • Danger
 Farekategori
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Skin corrosion/irritation Category 1 C
 Faresætning
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H412-Harmful to aquatic life with long lasting effects.
 Sikkerhedssætning
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P330-Rinse mouth.
  • P363-Wash contaminated clothing before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplerende oplysninger
  • MIXTURE LIST-Contain: Thiocyanic acid

For Research Use Only. Not for use in diagnostic procedures.

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