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Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
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Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers greater than 400 ng/μL of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights
- Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
- High concentration of DNA purified— >400 ng/μL
- Convenient—spin column format, centrifuge or vacuum protocols
- Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- Transfection of robust cell lines
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription
Specifications
Specifications
| Concentration | 400 ng/μL |
| Column Type | Spin Column |
| Format | Spin Column |
| Isolation Technology | Silica Column |
| Scale | Maxi |
| Sample Type | Bacteria |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| No. of Reactions | 25 Preps |
| Prep Scale | > 200 μg (Large-Scale) Plasmid DNA |
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Safety and Handling
- GeneJET Plasmid Maxiprep Kit
- Danger
- Skin corrosion/irritation Category 1 A
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H319-Causes serious eye irritation.
- H314-Causes severe skin burns and eye damage.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P373-DO NOT fight fire when fire reaches explosives.
- MIXTURE LIST-Contains : Sodium lauryl sulfate, Sodium hydroxide,acetic acid, Sodiumlauryl sulfate, Sodium hydroxide
For Research Use Only. Not for use in diagnostic procedures.
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