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Thermo Scientific™ GeneJET Gel Extraction and DNA Cleanup Micro Kit

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures.

111.00€ - 508.00€

Specifications

Column Type Spin Column
Format Spin Column
Isolation Technology Silica Column
Sample Type DNA, Gel Samples
For Use With (Application) Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
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Product Code Brand Quantity Price Quantity & Availability  
13293149
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Thermo Scientific™
K0832
250 Preps
508.00€
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13253149
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Thermo Scientific™
K0831
50 Preps
111.00€
Each
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Description

Description

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 μg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup

Thermo Scientific GeneJET Gel Extraction and DNA Cleanup Micro Kit is developed as 3 in 1 kit designed for rapid and efficient purification of DNA from PCR, enzymatic reaction mixtures, and DNA extraction from standard or low-melting point agarose gels run in either Tris acetate (TAE) or Tris borate (TBE) buffer. The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 μg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup

Highlights
Advanced column design—easy to open, load and handle
Yields concentrated DNA - Elute in 6 to 10 μL volume
High recovery - 90 to 100% recovery in the 100 to 3000 bp DNA fragment range
Fast—4 minute reaction mixture clean-up and 15 minute agarose gel purification protocols available
Efficient removal of reaction contaminants - including enzymes, proteins, primers dimers, dNTPs, and salts
Flexible—does not require precise gel slice quantification weighting

Applications
The purified DNA can be used in common downstream applications:
• Sequencing
• Restriction digestion
• PCR
• qPCR
• Labeling
• Ligation
• Cloning
In vitro transcription
• Blotting
In situ hybridization

Includes
• Binding Buffer
• Extraction Buffer
• Prewash Buffer (conc.)
• Wash Buffer (conc.)
• Elution Buffer
• DNA Purification Micro Column & Collection Tube

Specifications

Specifications

Spin Column
Silica Column
Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
GeneJET
DNA from Enzymatic Reactions (e.g. PCR)
Spin Column
DNA, Gel Samples
Not High-throughput Compatible (Manual)
Gel Extraction and DNA Cleanup Kit
15 min.
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.