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  5. Invitrogen™ Dynabeads™ SARS-CoV-2 Spike IgM ELISA Kit

Invitrogen™ Dynabeads™ SARS-CoV-2 Spike IgM ELISA Kit

Product Code. 17561825
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Quantity:
96 reactions
Unit Size:
Pack of 96
This item is not returnable. View return policy

Product Code. 17561825

Brand: Invitrogen™ 18010D

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Description

The Dynabeads™ SARS-CoV-2 Spike IgM ELISA Kit is a solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgM from serum and plasma samples. The product can be used both for qualitative and quantitative assays.

  • A solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgM from serum and plasma samples
  • The steps required for use are simply to pre-dilute the reagents, fill the wells of the KingFisher plastic plates, and push start on the instrument
  • After the run, the sample is transferred to a plate compatible with the absorbance reader and the OD is read at 450 nm
  • A DynaMag-96 well magnet can be used while still taking advantage of the faster binding kinetics of magnetic beads compared to the more traditional plate-bound ELISAs
  • Kit contains all buffers required, as well as calibrator antibodies, HRP-conjugated detection antibodies, substrate solution, beads, and stop solution sufficient for one 96-well plate
  • The intra-assay CV value is typically < 5% (range 2.4-3.9%), while the inter-assay CV is < 15% (range 3.1-14.4%)
  • Assay can be used both for qualitative and quantitative assays
  • One of the fastest ELISA assays for SARS-CoV-2 serology
  • Dynabeads facilitated automation limits hands-on time and improves reproducibility by reducing most operating errors
  • For research use only, not for use in diagnostic procedures
TRUSTED_SUSTAINABILITY

Specifications

Certifications/Compliance ISO 9001
Content And Storage • Dynabeads; SARS-CoV-2 Spike, 3 mL (1 mg/mL)
• Human SARS-CoV-2 IgM High Control antibody, 1 vial (lyophilized)
• Human SARS-CoV-2 IgM Low Control antibody, 1 vial (lyophilized)
• Human SARS-CoV-2 IgM Calibrator (medium control) antibody, 1 vial (lyophilized)
• Wash buffer (20X concentrated), 50 mL
• Assay buffer (20X concentrated), 50 mL
• Substrate solution (tetramethylbenzidine), 15 mL
• Stop solution, 15 mL
• Human SARS-CoV-2 IgM HRP Conjugate, 0.12 mL

Store at 2–8°C.
Detection Method Absorbance at 450 nm
Product Type SARS-CoV-2 ELISA IgM Kit
For Use With (Application) Binding of human IgM towards SARS-CoV-2 (Spike trimer)
For Use With (Equipment) KingFisher™ Flex or Apex Instruments, DynaMag™-96 Side Skirted, Magnetic 96-Well Separator, ELISA Plate Reader (450 nm)
Quantity 96 reactions
Shipping Condition Wet ice (2° to 8°C)
Diameter (Metric) Bead 2.7 μm
Material Monosized, superparamagnetic polystyrene magnetic beads
High-throughput Compatibility Yes, on KingFisher™ Flex or Apex instruments
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For research use only. Not for use in diagnostic procedures.

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