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Applied Biosystems™ Dynabeads™ MAX EPEC/VTEC O45 Kit
Description
The Dynabeads™ MAX EPEC/VTEC O45 Kit allows for the rapid, selective concentration of E. coli O45 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.
Improve detection sensitivity in PCR sample analysis
Reduce background in selective agar detection
Perform the method manually or automate using the BeadRetriever™ System
Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.
Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the BeadRetriever™ System.
Sensitivity
Following the Dynabeads™ MAX EPEC/VTEC O45 protocol, the presence or absence of one viable E. coli O45 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.
Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.
Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the BeadRetriever™ System.
Sensitivity
Following the Dynabeads™ MAX EPEC/VTEC O45 protocol, the presence or absence of one viable E. coli O45 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.
Specifications
Specifications
| Content And Storage | Store in refrigerator (2–8°C). DO NOT FREEZE. |
| Description | 150 μL output (automated prep), 100 μL output (manual prep) |
| Product Type | EPEC/VTEC Kit |
| For Use With (Equipment) | Dynabeads™ MPC™, KingFisher mL Food Protection Purification System |
| Product Line | Dynabeads |
| Quantity | 100 Reactions |
| Shipping Condition | Wet Ice |
For Testing of Food and Environmental Samples Only.
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