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Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM)
Stain live or fixed cells with this far-red DNA stain.
Brand: Thermo Scientific™ 62251
Additional Details : CAS : 9048-46-8 Weight : 1.24740kg
Description
Stain live or fixed cells with Thermo Scientific™ DRAQ5 Fluorescent Probe, a far-red DNA stain.
Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA, fluorescence microscopy and high-content imaging require a fluorescent label to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a fluorescent spectrum different than the other probes. The blue-fluorescent DNA-binding probes, Hoechst and DAPI are frequently used; however, these probes cannot be used when UV illumination is unavailable or other blue-emitting fluorescent probes are used. Therefore, nuclear probes that emit in a color other than blue is useful for cell identification and counting, and for determining nuclear morphology and DNA content.- Due to its far-red excitation and emission, the DRAQ5 Fluorescent Probe can be multiplexed with many other fluorophores and is ideal for cells expressing green fluorescent protein (GFP) fusion proteins
- Compatible with many existing protocols across a wide range of instrumentation platforms
- Emits in the far-red region, is lipophilic and crosses cell and nuclear membranes in live and fixed cells and tissues for rapid DNA staining
- Stain is water-soluble, supplied ready-to-use and does not require RNase, cell lysis, or a washing step, making it compatible with automation
- Because DNA staining is stoichiometric, the DRAQ5 Fluorescent Probe can be used for DNA content analysis in cell proliferation studies
Specifications
| Cell-permeant | |
| Store at 4°C. | |
| Fluorescence | |
| Fluorescence Microscope, High Content Analysis Instrument | |
| Cell-Permeant | |
| 647 nm | |
| Pierce™ | |
| 200 μL | |
| Nucleus, Cytosol, Cytoplasm |
| Red | |
| DRAQ5™ Fluorescent Probe Solution (5 mM) | |
| Cell Imaging | |
| Fluorescent Probe Solution | |
| 681 nm | |
| Liquid | |
| > 99% | |
| Soluble at working concentrations in water and typical buffers |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- DRAQ5 Fluorescent Probe Solution (5 mM)
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione
For Research Use Only. Not for use in diagnostic procedures.