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BD Biosciences™ DAPI Solution (RUO)

Product Code. 15823959
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1 mg
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15823959 1 mg Each
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Product Code. 15823959 Supplier BD Biosciences™ Supplier No. 564907

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Description

Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.

  • Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.
  • DAPI is predominantly impermeant to live cells, allowing it to be used as a viability dye in unfixed cells to discriminate intact from membrane-compromised cells.
  • Note, however, that high concentrations of the dye may still enter intact cells.
  • Additionally, DAPI may be used to analyze DNA content in fixed cells, or as a nuclear counterstain in imaging or flow cytometry.
  • When bound to double-stranded DNA, DAPI has an excitation wavelength maximum of 358 nm and an emission maximum of 461 nm.
  • DAPI is also well excited by the violet laser line (eg, 405 nm). Note that DAPI also binds RNA.
  • Under these conditions, DAPI emits maximally at 500 nm, but with less intensity than when bound to double-stranded DNA.
TRUSTED_SUSTAINABILITY

Specifications

Concentration 1.0 mg/mL
For Use With (Application) Flow cytometry, bioimaging, immunofluorescence
Quantity 1 mg
Storage Requirements Avoid multiple freeze-thaws of product. The product should be kept undiluted at -20°C for long term storage, and it may be kept undiluted at 4°C for short term storage.
Stain Type Nucleic Acid Stain
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