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Phosflow Lyse/Fix Buffer 5X, BD
For the lysing and fixing of whole blood for use with intracellular flow cytometry
Brand: BD Biosciences 558049
Additional Details : CAS : 50-00-0 Weight : 0.25000kg Transport : UN number : 2209 Chem class : 8 Pack group : III
Description
- Can be used for lysing red blood cells and for fixing leukocytes in one step, allowing investigators to rapidly inactivate kinases, phosphatases and/or proteases, so that the in vivo phosphorylation state of the cell can be examined
- Has been reported to preserve the light scattering properties of the cell
- May be used on human and mouse whole blood
- Whole blood lysis has been shown to be as effective as density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis
- In many cases, phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g. CD markers); however, not all cell surface markers, however, are compatible with the BD Phosflow Lyse/Fix Buffer
Specifications
| Room Temperature | |
| Lyse/Fix Buffer | |
| RUO |
| Liquid | |
| Clear | |
| 250mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- BD Phosflow Lyse/Fix Buffer
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Acute toxicity Category 4
- Carcinogenicity Category 1B
- Serious eye damage/eye irritation Category 2
- Germ cell mutagenicity Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity after repeated exposure Category 2
- Specific target organ toxicity Category 2
Hazard Statement
- H241-Heating may cause a fire or explosion.
- H302-Harmful if swallowed.
- H311-Toxic in contact with skin.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H331-Toxic if inhaled.
- H335-May cause respiratory irritation.
- H350-May cause cancer.
- H371-May cause damage to organs.
- H373-May cause damage to organs.
Precautionary Statement
- P201-Obtain special instructions before use.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
Supplemental information
- MIXTURE LIST-Contains : formaldehyde, diethylene glycol, methanol