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STEMCELL Technologies ArciTect™ Cas9-eGFP Nuclease
Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease (3 μg/μL) for the generation of double-strand breaks in CRISPR-Cas9 genome editing
Brand: STEMCELL Technologies 76006
Additional Details : Weight : 0.00110kg
Description
- ArciTect™ Cas9-eGFP Nuclease is a fusion protein consisting of enhanced green fluorescent protein (eGFP) and the wild-type Cas9 recombinant protein from Streptococcus pyogenes
- ArciTect™ Cas9-eGFP Nuclease contains a C-terminal-linked eGFP molecule that can be used for fluorescence-activated cell sorting of positive transfectants or other experiments requiring the visualization of Cas9
- ArciTect™ Cas9-eGFP Nuclease requires association with a guide RNA—eg ArciTect™ sgRNA (Catalog #200-0013) or a duplex composed of ArciTect™ tracrRNA (Catalog #76016) and ArciTect™ crRNA (Catalog #76010)—to form a ribonucleoprotein (RNP) complex
- This RNP complex creates double-strand breaks at site-specific locations in the genome
- ArciTect™ Cas9-eGFP Nuclease also contains a nuclear localization signal at the N-terminus, ensuring that the RNP complex translocates to the nucleus, thereby increasing the efficiency of genome editing
Specifications
| 100 μg | |
| Pluripotent Stem Cells |