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![p27[Kip1] Mouse, Unlabeled, Clone: 57, BD 50μg; Unlabeled](https://assets.fishersci.com/TFS-Assets/CCG/product-images/F882044.jpg-650.jpg)
![p27[Kip1] Mouse, Unlabeled, Clone: 57, BD 50μg; Unlabeled](https://assets.fishersci.com/TFS-Assets/CCG/product-images/F882045.jpg-650.jpg)
p27[Kip1] Mouse, Unlabeled, Clone: 57, BD
Mouse Monoclonal Antibody
Brand: BD Biosciences 610241
Additional Details : Weight : 0.01000kg
Description
Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGFβ-induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF-β in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF-β also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF-β treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro.
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Specifications
| p27[Kip1] | |
| Monoclonal | |
| 250μg/mL | |
| Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. | |
| Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. | |
| 50 μg | |
| Cell Biology | |
| Canine, Chicken, Frog, Human, Mouse, Rat | |
| IgG1 |
| Western Blot | |
| 57 | |
| Unconjugated | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Store undiluted at -20°C. |
For Research Use Only.