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Thermo Scientific™ Amplified IDEIA™ HP StAR™ Kit using Enzyme Immunoassay
Beskrivelse
Rapidly detect Helicobacter pylori antigens in human stool samples with this sanwich type enzyme immunoassay in microplate format.
Rapidly detect Helicobacter pylori antigen direct from fecal samples using the Thermo Scientific™ Amplified IDEIA™ HP StAR™ Kit using Enzyme Immunoassay. The test utilizes highly specific monoclonal antibodies combined with a polymer dextran conjugate to achieve high sensitivity and specificity. This in vitro qualitative enzyme immunoassay is intended to aid the diagnosis of Helicobacter pylori infection and to monitor eradication post-therapy in both adult patients and children.
Helicobacter pylori infection is the most common cause of gastritis and is involved in gastric ulcer, duodenal ulcer, and gastric adenocarcinoma. It is also the causative agent in a high percentage of dyspepsia cases.
H. pylori bacteria have adapted to live in the acidic environment of the stomach. The enzyme urease cleaves urea into ammonia and carbon dioxide, thus neutralizing the acid and enabling H. pylori to survive the bactericidal conditions of the stomach. The production of catalase and superoxide dismutase protects the bacteria from neutrophilic attack in the stomach mucosa1.
Use Amplified IDEIA HP StAR for the initial diagnosis of H.pylori infections as well as for monitoring eradication success four to six weeks after completion of eradication therapy and also for the diagnosis of reinfection.
The Amplified IDEIA™ Hp StAR™ test is a sandwich-type enzyme immunoassay using immunoassay amplification technology for the determination of H. pylori antigens in feces.
- Rapid results- within little over 70 minutes of receiving test sample
- Simple- ready-to-use reagents
- Excellent sensitivity and specificity- high quality, well-characterized antibodies used in the assay provide excellent sensitivity and specificity
- Easy-to- read results
- Long Shelf Life- typically 24 months
The wells of the microplate are coated with monoclonal antibodies specific for H. pylori antigens. Supernatant of a fecal suspension, as well as horseradish peroxidase (HRP) labelled monoclonal antibodies (Antibody Conjugate), are added to the wells in one step. During incubation, H. pylori antigens present in the sample bind to the antibodies on the microplate and to the HRP labeled antibodies thus forming a ‘sandwich complex’. The wells are washed in order to remove unbound Antibody Conjugate. A colourless single-component enzyme substrate (Tetramethylbenzidine – TMB) is added. Bound HRP oxidizes TMB to a blue coloured product. By adding the Stop Solution the colour changes to yellow. The intensity of the colour is determined spectrophotometrically.
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Tekniske data
Tekniske data
| Content And Storage | 2°C to 8°C |
| Description | Amplified IDEIA HP StAR™ Kit using Enzyme Immunoassay |
| Detectable Analytes | H. pylori Antigen |
| Sample Type | Fecal |
| Type | H. pylori Testing Kit |
| For Use With (Application) | Enzyme Immunoassay Detection |
| Includes | Antibody coated microwells, sample diuent, positive Control, negative control, conjugate, wash buffer concentrate, substrate, stop solution. |
| Quantity | 96 Wells |
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