missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Lysates
  5. Cell Line Lysates
  6. 16029615

Abnova ES-E14TG2a (Mouse pluripotent embryonic stem cell) whole cell lysate (nondenatured)

Product Code. 16029615
Click to view available options
Quantity:
50 μg
Unit Size:
50µg
This item is not returnable. View return policy

Product Code. 16029615

Brand: Abnova L045V2.50ug

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

  • Maintained in the undifferentiated state using specialized culturing protocols
  • Monitored for undifferentiated cell morphology
  • Analyzed using Flow Cytometry against specific cell marker
  • Coommasie Blue Staining of total cell lysate in SDS-PAGE

Western Blotting, Immunoprecipitation

Spécification

For Use With (Application) Immunoprecipitation, Western Blot
Tissue Embryonic
Description Whole cell lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 50 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Mouse
Concentration 4 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Afficher plus Afficher moins
Correction du contenu d'un produit

Veuillez fournir vos retours sur le contenu du produit en remplissant le formulaire ci-dessous.

Nom du produit

En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.

Merci de nous aider à améliorer notre site web. Votre commentaire a été soumis