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A-549 (human lung carcinoma) Whole cell lysate, Denatured; Abnova

Product Code. 16019575
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Quantity:
200 μg
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200µg
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Product Code. 16019575

missing translation for 'mfr': Abnova L025V1.200ug

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Description

Western Blotting

Specifications

For Use With (Application) Western Blot
Tissue Lung
Description Whole cell lysate (denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Host Species Human
Concentration 3 mg/mL
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Safety and Handling

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Product Identifier
  • A-549 (human lung carcinoma) whole cell lysate (denatured)
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Supplemental information
  • MIXTURE LIST-Contains : 2-mercaptoethanol
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