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BD Biosciences™ 488 EdU Click Proliferation Kit (RUO)

Product Code. 15864329
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50 Tests
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15864329 50 Tests Each
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Product Code. 15864329 Supplier BD Biosciences™ Supplier No. 565455

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Description

For analyzing DNA synthesis in cycling cells.

  • Provides a tool for analyzing DNA synthesis in cycling cells.
  • In this method, the thymidine analog EdU (5-ethynyl-2´-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle.
  • EdU can then be detected in fixed and permeabilized cells with a fluorophore-labeled azide.
  • The reaction of the fluorophore-labeled azide and EdU occurs via a copper-catalyzed click reaction between the azide moiety of the fluorophore and the alkyne moiety of EdU.
  • When co-stained with a DNA dye such as 7-AAD, PI, or DAPI, cell populations may be segmented by flow cytometry into the G0/G1-phases (2N DNA content, EdU-negative), S-phase (2N-4N DNA content, EdU-positive), or G2/M-phases (4N DNA content, EdU-negative).
  • Contains 6-FAM Azide, which is excited by the blue laser and has an excitation maximum of 496 nm and an emission maximum of 516 nm.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage EdU, 6-FAM Azide, Buffer Additive (10X) to be stored dry and protected from light at -20°C. Saponin-based Permeabilization and Wash Reagent (10X), Fixative, Catalyst, DMSO to be stored dry at 2 - 8°C or room temperature.
Description 50 Tests
Detection Method Flow Cytometry, Fluorophore
Includes 10 mg EdU, 130 μL 6-FAM Azide, 400 mg Buffer Additive (10X), 50 mL Saponin-based Permeabilization and Wash Reagent (10X), 5 mL Fixative, 2 mL Catalyst, 5 mL DMSO.
Quantity 50 Tests

Safety and Handling

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Product Identifier
  • 488 EdU Click Proliferation Kit
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Carcinogenicity Category 1A
  • Serious eye damage/eye irritation Category 1
  • Germ cell mutagenicity Category 2
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H318-Causes serious eye damage.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
  • H341-Suspected of causing genetic defects.
  • H350-May cause cancer.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P370+P378-In case of fire: Use CO2, powder or waterspray to extinguish.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : formaldehyde, coppersulphate
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