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Description
Includes
Precipitant, 250mL; Co-precipitant, 250mL; Copper solution, 50mL; Color reagent A, 2 x 250mL; Color reagent B, 5mL; BSA standard (2mg/mL), 5mL
Designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques. Works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein.
- Works in the presence of 2% SDS, 1% DTT, 8M urea, 2M thiourea, 4% CHAPS, 2% Pharmalyte™, and 2% IPG Buffer
- Quantitatively precipitates proteins while leaving interfering substances behind
- Linear response in the range of 0 to 50μg protein, with sample volumes of 1 to 50μL
- Assay time: one hour
2-D electrophoresis, SDS-PAGE, IEF
Specifications
Specifications
| For Use With (Application) | 2D Electrophoresis, SDS-PAGE, IEF |
| Includes | 250mL Precipitant, 250mL Co Precipitant, 50mL Copper Solution, 2 x 250mL Color reagent A, 5mL Color reagent B, 5mL BSA standard (2mg/mL) |
| Sample Volume | 1 to 50μL |
| Content And Storage | 4° to 8°C |
| Product Type | 2D Quant kit |
| Quantity | 500 Assays |
Safety and Handling
- 2-D Quant Kit, 500 assays
- Danger
- Skin corrosion/irritation Category 1 A
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- Serious eye damage/eye irritation Category 2
- LONG-TERM AQUATIC HAZARD Chronic 2
- ACUTE AQUATIC Acute 1
- LONG-TERM AQUATIC HAZARD Chronic 1
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H335-May cause respiratory irritation.
- H319-Causes serious eye irritation.
- H411-Toxic to aquatic life with long lasting effects.
- H400-Very toxic to aquatic life.
- H410-Very toxic to aquatic life with long lasting effects.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P501-Dispose of contents/container to in accordance with all local, regional,national and international regulations.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P331-Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P310-Immediately call a POISON CENTER/doctor/.
- P405-Store locked up.
- MIXTURE LIST-Contain: sodium carbonate, Cholan-24-oic acid, 3,12-dihydroxy-, monosodiumsalt, monohydrate, (3.alpha., 5.be
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