Classical and Basal Cell Culture Media
An extensive portfolio of high-quality classical and basal media formulations such as MEM, DMEM, RPMI, in convenient packaging configurations.
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Product Code 7058061
Cytiva HyClone™ RPMI 1640 Media
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Product Code 7058065
Cytiva HyClone™ Dulbecco's Modified Eagles Medium
Product Code 11470425
MP Biomedicals™ RPMI 1640 Medium, 1X Liq., w/o L-Glutamine and Glucose
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Product Code 11430425
MP Biomedicals™ Dulbecco's MEM, 1X Liq., w/o L-Glut., i-Inositol
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FAQ
Basal media are basic nutrient solutions that provide essential nutrients for cell growth. They are often used as a starting point for customizing cell culture conditions but are not sufficient for optimal cell growth by themselves and usually require supplementation. Examples include DMEM, RPMI-1640 and MEM (Minimum Essential Medium).
Complete media are basal media supplemented with additional components like fetal bovine serum (FBS), growth factors, and hormones to support optimal cell growth. An example is DMEM with 10% FBS. Complete media are ready-to-use solutions tailored for the optimal growth of specific cell lines or primary cells, providing all the nutrients and growth factors necessary for cell culture.
The different types of cell culture media include:
- Basal Media: Basic nutrient solutions (e.g., DMEM, RPMI-1640)
- Serum-Supplemented Media: Basal media with added serum (e.g., DMEM + 10% FBS)
- Serum-Free Media: Formulated without serum, with specific growth factors (e.g., RPMI-1640 with specific supplements)
- Chemically Defined Media: Composed of known quantities of all ingredients (e.g., Hybridoma-SFM)
- Specialized Media: Tailored for specific cell types or applications (e.g., Neurobasal Medium)
- Stem Cell Media: Designed for stem cell growth and maintenance (e.g., mTeSR1)
- 3D Culture Media: For culturing cells in three-dimensional structures (e.g., Matrigel)
When selecting classical and basal cell culture media, consider the following:
- Cell Type Requirements: Ensure the media meet the specific nutritional and environmental needs of the cell type
- Growth Factors and Supplements: Determine if additional growth factors, hormones, or supplements are needed
- Serum Use: Decide whether to use serum-supplemented or serum-free media based on experimental needs
- Formulation Consistency: Choose media with consistent and reproducible formulations to ensure experimental reliability
- Compatibility: Ensure the media are compatible with the intended downstream applications and assays
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