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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,613)
Magnetic Affinity Separation Particles (179)
Spin Columns and Gravity-flow Columns (119)
Gel Filtration Media and Columns (115)
Protease Inhibitors and Phosphatase Inhibitors (111)
Miscellaneous Immobilization Supports (110)
Cell Lysis Detergents (71)
Affinity Gels (35)
Biotin-Binding Affinity Resins and Beads (30)
Miscellaneous Immobilized Affinity Ligands (25)
In vitro Protein Expression Systems (20)
Cell Lysis Enzymes (19)

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1 – 15 of 1,514 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2
Promotions Available

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

EDGE
Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor
Form Liquid
Product Type Protease Inhibitor Cocktail
Reagent Type Protease Inhibitor
Product Line Halt
3

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
4
Promotions Available

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

EDGE
5
Promotions Available

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Shipping Condition Wet Ice
Assay End-point Chromogenic Assay
Type Endotoxin Quantitation Kit
Target Endotoxins
Content And Storage • Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromogenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.
Format Kit
Assay Sensitivity 0.01 EU/mL
Assay Range 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line Pierce™
Detection Method Colorimetric
6
Promotions Available

Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

EDGE
Content And Storage Upon receipt store product at 4°C. Do not freeze.
Inhibitors Phosphatase Inhibitor
Form Liquid
Product Type Phosphatase Inhibitor Cocktail
Reagent Type Phosphatase Inhibitor
Product Line Halt
7

Proteintech ChromoTek GFP-Trap™ Magnetic Agarose

The ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins.

Target AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
Content And Storage Upon receipt store at 4°C. Do not freeze.
Binding Capacity 10 μL slurry bind more than 8 μg of recombinant GFP
Label or Dye Magnetic Agarose
For Use With (Application) IP, CoIP, ChIP, RIP
Species Alpaca
8
Promotions Available

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

EDGE
Buffer Lysis Buffer
Inhibitors Protease Inhibitor, Phosphatase Inhibitor
Description Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
Form Liquid
Product Type Protease and Phosphatase Inhibitor Cocktail
Reagent Type Protease Inhibitor, Phosphatase Inhibitor
Product Line Halt
9
Promotions Available

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

EDGE
dimMatrix Magnetic Agarose
10
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
11

IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

12
Promotions Available

Thermo Scientific™ DNase I Solution (2500 U/mL)

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

EDGE
13
Promotions Available

Thermo Scientific™ Pierce™ Disposable Columns, 2 mL

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

EDGE
14

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

EDGE
Stationary Phase Proprietary Ligand
Type Spin Column
Target Endotoxin Removal
Content And Storage Upon receipt store at 4°C.
Format Spin Column
Form Liquid
Column Type Affinity, Cellulose Resin
Formulation 22mL-capacity centrifuge columns containing 1mL resin
Capacity (Metric) Up to 2,000,000 EU/mL
Product Line Pierce
15
Promotions Available

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.

EDGE
Content And Storage Store at room temperature.
Column Type Empty Column, Polyethylene Filter
Product Line Pierce