Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Thermo Scientific™ 1.1 mL Microtube System, Racked

Pre-racked 1.1mL-tall microtubes boxed to match a standard 96-well microplate footprint for multichannel pipetting or automated workstations.

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientificâ„¢ 6X DNA Loading Dye.

Thermo Scientific™ Spectra™ Multicolor Low Range Protein Ladder

A mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.

Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.

Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)

Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.

Hoefer™ Blot Module for MiniVE Mini Vertical Electrophoresis Unit

Can be used to transfer two minigels inside the MiniVE tank

Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

Thermo Scientific™ Western Blotting Filter Paper, 0.83 mm thick, 7 x 8.4 cm

Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Acrylamide, +99%, for biochemistry, electrophoresis grade, ACROS Organics™

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 MDL Number: MFCD00008032 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N Synonym: Ethylenecarboxamide, 2-Propenamide PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Thermo Scientific™ Pierce™ Unstained Protein MW Marker

A mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Pierce™ Prestained Protein MW Marker

A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Acrylamide / N,N'-Methylenebisacrylamide 37.5:1, for biochemistry, 40% mix solution, ACROS Organics™

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 MDL Number: MFCD00080848 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ Blocker™ BLOTTO in TBS

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Fisher BioReagents™ EZ-Run™ Rec Protein Ladder

Mixture of 14 highly purified recombinant proteins optimized for precise sizing of proteins by SDS-PAGE and Western blotting

Alfa Aesar™ Acrylamide/Bisacrylamide 37.5:1, 40% soln

Premixed solution for preparing nucleic acid PAGE gels

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

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