Electrophoresis, Western Blotting and ELISA
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Agarose (Low-EEO/Multi-Purpose/Molecular Biology Grade), Fisher BioReagents
CAS: 9012-36-6 Molecular Formula: C12H18O9
Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Thermo Scientific™ Agarose LE, Molecular Biology Grade, Ultrapure, Thermo Scientific™
CAS: 9012-36-6 MDL Number: MFCD00081294
Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ P 0.45μm PVDF Membrane
Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.
Thermo Scientific™ 1.1 mL Microtube System, Racked
Pre-racked 1.1mL-tall microtubes boxed to match a standard 96-well microplate footprint for multichannel pipetting or automated workstations.
Thermo Scientific™ DNA Gel Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Thermo Scientific™ Spectra™ Multicolor Low Range Protein Ladder
A mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.
Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder
A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.
Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder
A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)
Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
Hoefer™ Blot Module for MiniVE Mini Vertical Electrophoresis Unit
Can be used to transfer two minigels inside the MiniVE tank
Agarose (Broad Separation Range for DNA/RNA/Genetic Analysis Grade), Fisher BioReagents
CAS: 9012-36-6 Molecular Formula: C12H18O9
Invitrogen™ TMB Chromogen Solution (for ELISA)
May be used as a substrate for horseradish peroxidase (HRP) in ELISAs
Thermo Scientific™ Western Blotting Filter Paper, 0.83 mm thick, 7 x 8.4 cm
Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.
Thermo Scientific™ Pierce™ DAB Substrate Kit
Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.
Thermo Scientific™ PageRuler™ Unstained Protein Ladder
A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
Acrylamide, +99%, for biochemistry, electrophoresis grade, ACROS Organics™
CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 MDL Number: MFCD00008032 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N Synonym: Ethylenecarboxamide, 2-Propenamide PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N
Thermo Scientific™ Pierce™ Unstained Protein MW Marker
A mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
Thermo Scientific™ Pierce™ Prestained Protein MW Marker
A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls
Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Acrylamide / N,N'-Methylenebisacrylamide 37.5:1, for biochemistry, 40% mix solution, ACROS Organics™
CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 MDL Number: MFCD00080848 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N
Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution
Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ Blocker™ BLOTTO in TBS
Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.
Fisher BioReagents™ EZ-Run™ Rec Protein Ladder
Mixture of 14 highly purified recombinant proteins optimized for precise sizing of proteins by SDS-PAGE and Western blotting
Alfa Aesar™ Acrylamide/Bisacrylamide 37.5:1, 40% soln
Premixed solution for preparing nucleic acid PAGE gels
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis