Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Thermo Scientific™ DNA Gel Loading Dye (6X)

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ 1.1 mL Microtube System, Racked

Thermo Scientific™ 1.1 mL Microtube System, Racked

Pre-racked 1.1mL-tall microtubes boxed to match a standard 96-well microplate footprint for multichannel pipetting or automated workstations.

Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ Spectra™ Multicolor Low Range Protein Ladder

Thermo Scientific™ Spectra™ Multicolor Low Range Protein Ladder

A mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.

Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

A mixture of 8 purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.

Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)

Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)

Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.

Hoefer™ Blot Module for MiniVE Mini Vertical Electrophoresis Unit

Hoefer™ Blot Module for MiniVE Mini Vertical Electrophoresis Unit

Can be used to transfer two minigels inside the MiniVE tank

Thermo Scientific™ Pierce™ DAB Substrate Kit

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Thermo Scientific™ Pierce™ Unstained Protein MW Marker

Thermo Scientific™ Pierce™ Unstained Protein MW Marker

A mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Western Blotting Filter Paper, 0.83 mm thick, 7 x 8.4 cm

Thermo Scientific™ Western Blotting Filter Paper, 0.83 mm thick, 7 x 8.4 cm

Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.

Thermo Scientific™ Pierce™ Prestained Protein MW Marker

Thermo Scientific™ Pierce™ Prestained Protein MW Marker

A mixture of six blue-stained natural proteins (20 to 120kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Acrylamide, +99%, for biochemistry, electrophoresis grade

Thermo Scientific™ Acrylamide, +99%, for biochemistry, electrophoresis grade

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.08 MDL Number: MFCD00008032 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N Synonym: Ethylenecarboxamide, 2-Propenamide PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: NC(=O)C=C

Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Acrylamide / N,N'-Methylenebisacrylamide 37.5:1, for biochemistry, 40% mix solution

Thermo Scientific™ Acrylamide / N,N'-Methylenebisacrylamide 37.5:1, for biochemistry, 40% mix solution

Acrylamide / N,N'-Methylenebisacrylamide, CAS # 79-06-1, 7732-18-5, 110-26-9, 79-06-1, is a water soluble compound that cross-links to produce polyacrylamide gels that are widely used in chromatography and the electrophoretic separation of nucleic acids and proteins.

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ Acrylamide/Bisacrylamide Solution (40%, 37.5:1)

Thermo Scientific™ Acrylamide/Bisacrylamide Solution (40%, 37.5:1)

Acrylamide/Bisacrylamide Solution (40%, 37.5:1), also known as bioAcryl-P (40%, 37.5:1), is a premixed solution containing acrylamide and N,N'-methylenebisacrylamide (in a ratio of 37.5:1, respectively) used for preparing protein DNA gels.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Thermo Scientific™ Blocker™ BLOTTO in TBS

Thermo Scientific™ Blocker™ BLOTTO in TBS

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Merck Immobilon™-P PVDF Membrane

Merck Immobilon™-P PVDF Membrane

Hydrophobic PVDF Transfer Membrane for western blotting

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

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