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Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nco I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | DreamTaq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ AarI (2 U/μL)
Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | AarI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer AarI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 2 U/μL |
Thermo Scientific™ GeneJET Genomic DNA Purification Kit
Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
| Column Type | Spin Column |
|---|---|
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Thermo Scientific™ Eco32I (EcoRV) (10 U/μL)
The Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV).
| Concentration | 10 U/μL |
|---|
Applied Biosystems™ TaqPath™ ProAmp™ Master Mix
TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. |
| InhibitorToleranceSimple | Very High |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Multiplex Capability | Up to 3-plex, Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Taq DNA Polymerase |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
| GC-Rich PCR Performance | High |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Product Type | Universal Master Mix II |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Product Line | TaqMan |
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | phi29 DNA Polymerase |
| Product Type | Stand-alone enzyme |
| Reaction Time | 4 hours |
| Concentration | 10 U/μL |
| Optimal Reaction Temperature | 30°C |
| For Use With (Application) | MDA-WGA,RCA |
Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Quantity | Each |
| Format | Kit |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |