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ABI Applied Biosystems (128)
Affymetrix eBioscience (3)
Gibco (2)
Invitrogen (6)
Thermo Scientific (934)
Thermo Scientific ABgene (45)

Research Category

Drug Development (4)
Scarless Cloning (62)
Traditional Cloning (460)

Sensitive to Heat Inactivation

No (68)
Yes (437)

Optimal Reaction Temperature

30°C (9)
37°C (445)
50°C (2)
55°C (21)
65°C (25)

Methylation Sensitivity

CpG Methylation-Sensitive (239)
Dam Methylation-Sensitive (37)
Dcm Methylation-Sensitive (22)
Not CpG Methylation-Sensitive (289)
Not Dam Methylation-Sensitive (458)
Not Dcm Methylation-Sensitive (445)

Reaction Speed

<2 hr. (2)
15 to 30 min. (3)
15 to 30 sec./kb (8)
30 min. (15)
60 min. (9)
8 hr. (1)
Fast (76)
Fast or Standard (34)
Standard (83)

Product Line

Applied Biosystems (2)
EpiJET (5)
FastDigest (203)
RiboLock (3)
Tango (1)
TheraPure (15)

1 – 15 of 746 results

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan
Detection Method	Primer-probe

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Thermostability	Not Applicable
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqMan

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2X
For Use With (Application)	Standard PCR,High-fidelity PCR

Thermo Scientific™ FastDigest BglII

Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Bgl II
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	FastDigest

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Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nco I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Pricing and Availability
Specifications

Hot Start PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
For Use With (Application)	Hot-start PCR

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Phusion Plus DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>100X
Size (Final Product)	20 kb or less
Reaction Format	Ready to use master mix
Reaction Speed	15 to 30 sec./kb
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Pricing and Availability
Specifications

Direct PCR Reagents and Kits

Product Type	Multiplex Kit
For Use With (Equipment)	3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type	DNA
Technique	SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line	SNaPshot
Detection Method	Primer-probe

Thermo Scientific™ AarI (2 U/μL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	AarI
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	Unique Buffer (10X Buffer AarI)
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	2 U/μL

Thermo Scientific™ HindIII (10 U/μL)

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

Pricing and Availability
Specifications

Restriction Enzymes

Concentration	10 U/μL

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing and Availability
Specifications

Real Time PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type	RNA
Reaction Speed	Standard
Product Line	AgPath-ID, Ambion

Thermo Scientific™ Eco32I (EcoRV) (10 U/μL)

The Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV).

Pricing and Availability
Specifications

Restriction Enzymes

Concentration	10 U/μL

Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Content And Storage	Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Genotyping
PCR Method	qPCR
Multiplex Capability	Up to 3-plex, Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath

Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

Real Time PCR Master Mixes

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

Thermo Scientific™ NcoI (10 U/μL)

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).

Pricing and Availability
Specifications

Restriction Enzymes

Concentration	10 U/μL

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