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Thermo Scientific™ DH10B Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.

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Competent Cells

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2X
For Use With (Application)	Standard PCR,High-fidelity PCR

Thermo Scientific™ T4 DNA Ligase (5 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Pricing and Availability
Specifications

Modifying Enzymes

Product Type	T4 DNA Ligase
Enzyme	DNA Ligase
Compatible Buffer	10X T4 DNA Ligase Buffer
Concentration	5 U/μL

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Thermo Scientific™ PCR Master Mix (2X)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.

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Specifications

Standard PCR Reagents and Kits

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
Concentration	2X
Starting Material	DNA
For Use With (Application)	Standard PCR

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Phusion Plus DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>100X
Size (Final Product)	20 kb or less
Reaction Format	Ready to use master mix
Reaction Speed	15 to 30 sec./kb
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Thermo Scientific™ GeneJET Genomic DNA Purification Kit

Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.

Pricing and Availability
Specifications

DNA Extraction and Purification

Column Type	Spin Column
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing and Availability
Specifications

Real Time PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type	RNA
Reaction Speed	Standard
Product Line	AgPath-ID, Ambion

Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Content And Storage	Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Genotyping
PCR Method	qPCR
Multiplex Capability	Up to 3-plex, Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath

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Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

Real Time PCR Master Mixes

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 10⁶ Da isolated from E.coli.

Pricing and Availability

DNA Cloning and Mapping and Mutagenesis

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Pricing and Availability
Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Content And Storage	• RevertAid Reverse Transcriptase • RiboLock RNase Inhibitor • 5X Reaction Buffer • dNTP Mix • Oligo(dT)₁₈ Primer • Random Hexamer Primer • Control GAPDH RNA • Forward GAPDH Primer (10 μM) • Reverse GAPDH Primer (10 μM) • Nuclease-free water Store at –20°C.
Reverse Transcriptase	RevertAid
Final Product Type	First-Strand cDNA
For Use With (Application)	RT-PCR
Format	Kit
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Pricing and Availability
Specifications

Direct PCR Reagents and Kits

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Product Type	High-Fidelity DNA Polymerase
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Standard PCR,High-fidelity PCR

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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq DNA Polymerase
Format	Tube
Product Type	Fast Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Product Line	AmpErase, TaqMan

Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Pricing and Availability
Specifications

Modifying Enzymes

Polymerase	DNA Polymerase I
Product Type	Klenow Fragment
Compatible Buffer	Restriction Enzyme, PCR, RT Buffer
Concentration	5 U/μL

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