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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 48 results
1

Cytiva Protein G HP SpinTrap™ Columns

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns

EDGE
3

BD Recombinant Human β2 Microglobulin

Recombinant protein expressed in insect cells and purified by a combination of ion-exchange and gel-filtration methods

4

Cytiva HiTrap™ Protein L Prepacked Columns

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

Particle Size 85μm
Ligand Type Recombinant Protein L (E. coli), mammalian free
Flow Rate <500 cm/hr.
Height (Metric) 20mm
Content And Storage 2° to 8°C
pH Range 2 to 10
Product Type HiTrap Protein L column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs
Support Type Highly Cross-linked Agarose
Max. Pressure 5 bar
Height (English) 0.78 in.
5

Cytiva Protein G HP MultiTrap™ Plates

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates

6

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
7

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
8

Cytiva GSTPrep™ FF 16/10 Column

Fast and easy one-step, scale-up purification of GST-tagged recombinant proteins

9

Thermo Scientific™ Pierce™ Glutathione Agarose

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Stationary Phase Glutathione
Type Agarose
Content And Storage Upon receipt store at 4°C.
Format Bottle
Protein Form Recombinant
Column Type Affinity, Agarose Resin
Capacity (Metric) 40 mg Purified Recombinant GST Protein Binding per mL Resin
Product Line Pierce
10

Cytiva Capto™ L Chromatography Medium

Capto L is a BioProcess chromatography medium (resin) for the capture of antibodies and antibody fragments.

Particle Size 85μm
Ligand Type Recombinant Protein L (E. coli), mammalian free
Flow Rate <500 cm/hr.
Height (Metric) 200mm
Content And Storage 2° to 8°C
pH Range 2 to 10
Product Type Capto L chromato graphy media
For Use With (Application) High binding capacity, low ligand leakage and selectivity for a broad range of Antibody fragments reduces process time and amount of medium
Support Type Highly Cross-linked Agarose
Height (English) 7.87 in.
11

Cytiva His MultiTrap™ 96-well Filter Plates

Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged proteins

Type 96-well Filter Plate
Content And Storage 4°C to 30°C
Description His MultiTrap FF and His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC)
pH Range 3 to 12
Quantity 4 x 96-well Filter Plates
Material Polypropylene, Polyethylene
12

Cytiva Chelating Sepharose™ Fast Flow Medium

Use for purifying native and recombinant proteins and peptides that have an affinity for metal ions. Chelating Sepharose™ Fast Flow Medium provides robust performance and regulatory support.

13

Cytiva HisTrap™ FF Columns: 5 x 5mL

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

14

Cytiva MBPTrap™ HP Prepacked Columns

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

EDGE
Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 2° to 8°C
Bed Volume 1mL
pH Range >7
Column Type Ion Exchange Column
Material Polypropylene
For Use With (Application) Purification of MBP tagged Proteins
Max. Pressure 3 bar
Particle Size 34μm
Ligand Type Dextrin
Product Type Dextrin Sepharose HP prepacked column
Support Type 6% Highly Cross-linked Agarose
Height (English) 0.98 in.
15

Thermo Scientific™ CaptureSelect™ FcXL Ligand Leakage ELISA

Thermo Scientific™ CaptureSelect™ FcXL Ligand Leakage ELISA is designed to detect possible leached ligand of antibodies or antibody fragments purified using CaptureSelect FcXL Affinity Matrix.

Assay ELISA
Type ELISA Kit
Target CaptureSelect FcXL Ligand
Content And Storage Store in freezer (-5°C to -30°C).
Format Kit
For Use With (Equipment) Microplate Reader
Product Line CaptureSelect