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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (263)
Gel Filtration Media and Columns (93)
Affinity Gels (34)
Spin Columns and Gravity-flow Columns (22)
Biotin-Binding Affinity Resins and Beads (5)
Magnetic Affinity Separation Particles (5)
Protease Inhibitors and Phosphatase Inhibitors (1)

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1 – 15 of 218 results
1

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

EDGE
2

Cytiva Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.

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3

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

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4

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

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5

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

EDGE
Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl
6

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
7

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

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8

Cytiva CNBr-Activated Sepharose™ Medium

Use for coupling antibodies or other large proteins containing -NH2 groups to the Sepharose media. CNBr-Activated Sepharose™ Medium is a pre-activated media perfect for custom affinity applications.

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9

Cytiva Protein G Sepharose™ 4 Fast Flow Media

Rapid processing of large volumes, recoveries of 70 to 90%. Cytiva Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.

EDGE
Particle Size 90 μm
Content And Storage 2°C to 8°C
Matrix 4% Cross Linked Agarose
Ligand Recombinant Protein G lacking albumin binding region
Ligand Concentration 2mg Protein G/mL Medium
pH 3 to 9
For Use With (Application) Used in a range of diagnostic and research applications
10

Cytiva HiLoad™ 16/600 Superdex™ 200 pg

Use for separating MAbs from dimers and contaminants. Cytiva HiLoad™ 16/600 Superdex™ 200 pg is ideal for high-resolution preparative gel filtration chromatography.

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11

Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Particle Size 45 to 165 μm
Type Pre-packed Column
Content And Storage 4°C to 30°C
pH Range 3 to 12
Material Polypropylene
Support Type 6% Cross-linked Agarose
12

Cytiva Tricorn™ Filter Kit

Used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Cytiva Tricorn™ Filter Kit includes top and bottom adapters, bottom filters, and EPDM O-rings, five of each

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13

Cytiva MBPTrap™ HP Prepacked Columns

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

EDGE
Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 2° to 8°C
Bed Volume 1mL
pH Range >7
Column Type Ion Exchange Column
Material Polypropylene
For Use With (Application) Purification of MBP tagged Proteins
Max. Pressure 3 bar
Particle Size 34μm
Ligand Type Dextrin
Product Type Dextrin Sepharose HP prepacked column
Support Type 6% Highly Cross-linked Agarose
Height (English) 0.98 in.
14

Cytiva HiTrap™ MabSelect™ SuRe Prepacked Columns

Use these columns in small-scale MAb purification in a continuous bioprocess. Cytiva Life Sciences™ HiTrap™ MabSelect™ SuRe (Superior Resistance) Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Binding Capacity ≈35mg Human lgG/mL at 2.4 min. residence time
For Use With (Application) Chromatography System, Manual
15

Cytiva Protein G HP SpinTrap™ Columns

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns

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