Perform DNA extractions with this phenol:chloroform:isoamyl alcohol mixture (25:24:1), accompanied by a buffer to equilibrate the solution at either pH 6.7 or pH 8.0.
Sodium Phosphate Buffer is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. It occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4).