DNA Extraction and Purification
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Applied Biosystems™ PrepSEQ™ Lysis Buffer
PrepSEQ Lysis Buffer is a component of the PrepSEQ Express Nucleic Acid Extraction Kit, offered separately here for those kit users who do large-quantity nucleic acid extractions and would benefit from additional lysis buffer.
Applied Biosystems™ MagMAX CORE Nucleic Acid Purification Kit + Mechanical Lysis Module
The MagMAX™ CORE Mechanical Lysis Module Bundle includes a MagMAX™ CORE Mechanical Lysis Module and MagMAX™ CORE Nucleic Acid Purification Kit. The Mechanical Lysis Module is also available separately.
Applied Biosystems™ MagMAX™ CORE Mechanical Lysis Module & Glass Microbeads Bundle with CORE Chemistry
The MagMAX™ CORE Mechanical Lysis Module & Glass Microbeads Bundle with CORE Chemistry includes MagMAX™ CORE Glass Microbeads, a MagMAX™ CORE Mechanical Lysis Module, and a MagMAX™ CORE Nucleic Acid Purification Kit.
Cytiva Tissue and Cells genomicPrep Mini Spin Kit
Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures
| Includes | Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card |
|---|---|
| Description | illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce |
| Product Type | illustra tissue and cells genomicPrep Mini Spin Kit |
| Purity | 1.75 |
| Sample Volume | 5-50 mg |
| For Use With (Application) | Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR |
| Test Time | 90 min[Animal Tissue], 45 min[Cultured cells] |
Cytiva Single Cell GenomiPhi™ DNA Amplification Kit
Amplify whole genome DNA from as little as a single cell. Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
| Includes | Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL |
|---|---|
| Content And Storage | Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. |
| Description | Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference. |
| Product Type | illustra Single Cell GenomiPhi DNA Amplification Kit |
| Final Product Type | illustra™ Single Cell GenomiPhi™ DNA Amplification Kit |
| Size | >10kb |
| For Use With (Application) | Genomic DNA Amplification |
| Test Time | 2 to 4 hours |
MP Biomedicals™ MPure™ Cultured Cell DNA Extraction Kit
For extraction of genomic DNA from cultured cells in monolayer or suspension and from buffy coat
DNA Extract All Reagents Kit
Provides PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues, in 5 minutes
| Content And Storage | Store at 2–8°C. |
|---|---|
| Form | Liquid |
| Reagent Type | Cell Lysis Buffer |
| Test Time | 5 min |
| Product Line | Extract All |
Macherey-Nagel™ NucleoSpin™ Tissue, Mini kit for DNA from cells and tissue
Mini spin kit for purification of DNA from a broad range of samples
Macherey-Nagel™ NucleoSpin™ cfDNA Midi Kit for cell-free DNA from plasma
Isolation of cell-free DNA from up to 5 mL blood plasma, utilizing the NucleoVac 96 Vacuum Manifold (sold separately, MPN 740681). The kit is designed for high recovery of circulating fragmented DNA ≥ 50 bp from human EDTA plasma.
| No. of Reactions | 48 |
|---|---|
| Format | Midi Spin Column |
| For Use With (Equipment) | Vacuum (e.g. NucleoVac 96 Vacuum Manifold and vacuum pump) |
| Final Product Type | Cell-free DNA |
| Sample Type | Blood Plasma |
| Isolation Technology | Silica Membrane Technology |
| Purity | Fragment size: ≥ 50 bp |
| Sample Volume | 1 - 5 mL |
| Yield | Depends on sample, typically: 0.1 - 100 ng DNA/mL plasma |
| Elution Volume | Elution volume: 200 μL (140 μL final eluate) |
| For Use With (Application) | DNA isolation from blood plasma |
| Test Time | 90 min/24 preps |
Macherey-Nagel™ NucleoSpin™ Tissue Columns, Mini columns for DNA from cells and tissue
Mini spin kit for purification of DNA from a broad range of samples.
| Description | Columns for purification of DNA from a broad range of samples |
|---|---|
| Final Product Type | DNA |
| Size | 200 bp to 50 kbp |
| Purity | A260/A280 1.7 - 1.9 |
| Sample Volume | <25 mg human/animal tissue, 102-107 cultured cells |
| Key Functions | DNA purification |
| For Use With (Application) | Genotyping, Multiplex PCR, Next Generation Sequencing |
| Source | Animal & Human tissue, cells |
| Test Time | 20 min/prep (excl. lysis) |
| Detection Method | Silica-membrane Technology |
| Includes | NucleoSpin Tissue Columns |
| Target | DNA |
| Format | Mini Spin Column |
| For Use With (Equipment) | Centrifugation, Vacuum |
| Capacity | 60 μg |
| Shipping Condition | Room Temperature |
| Content And Storage | 18°C to 25°C |
| Isolation Technology | Silica Membrane Technology |
| Column Type | Mini spin column |
| Cell Type | Animal & Human tissue, cells |
| Product Type | DNA purification columns |
| Sample Type | Animal and Human Tissue,Cells |
| Yield | 20 to 35 μg |
| Technique | Silica-membrane technology |
| Shelf Life | 27 months after production |
| Nucleic Acid Type | DNA |
| Elution Volume | 60 to 100 μL |
Merck Replacement Solutions for Montage™ BAC96 Miniprep Kit and Montage Plasmid Miniprep96 Kit
Suitable for cell resuspension for plasmid & BAC minipreps
| Quantity | 500 mL |
|---|
MP Biomedicals™ Gnome™ DNA Isolation Kit
Gnome™ DNA Isolation Kit offers a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types.
| Includes | Cell Lysis/Denaturing Solution, RNase Mixx, Protease Mixx, Salt Out Mixture, Cell Suspension Solution |
|---|---|
| Format | Kit |
| Product Type | Gnome™ DNA Isolation Kit |
| Sample Type | Blood,Tissues,Yeast,Bacteria,Plant |
| pH | Cell Lysis/Denaturing Solution pH range: 6.2 to 6.9, Salt-out Mixture pH range: 6.0 to 6.8, Cell Suspension Solution pH range: 8.0 to 8.2 |
| Sample Volume | 20 to 50 mg wet weight bacterial cell pellet, Up to 250 mg plant tissue, 2 to 5 x 107 nucleated white blood cells, 50 to 250 mg mammalian tissue, ∼1 to 5 x 108 yeast cell pellet |
| Yield | 100 μg |
QIAGEN QIAcard FTA DMPK
For collection, stabilization, processing, transport and archiving of biological samples. 4 sample areas per card.
| Includes | 100 cards |
|---|---|
| Format | Card |
| For Use With (Application) | For protein-based biomolecules, on-substrate cleanup has potential to convey greater analyte stability, requires fewer experimental animals |
MP Biomedicals™ TeenPrep™ Lysing Matrix D Tubes
TeenPrep™ Lysing Matrix D Tubes contain 1.4 mm zirconium-silicate spheres and are used to grind soft animal and plant tissues, cultured cells and insects/worms.
| Matrix | Lysing Matrix D |
|---|---|
| Bead Type | 1.4 mm Zirconium-silicate Spheres |
| Sample Type | Animal Tissues,Plants,Cultured Cells,Insects,Worms |
| DNase | Dnase-free |
| For Use With (Application) | Nucleic Acid Isolation and Purification, Homogenization |
| RNase | Rnase-free |
Thermo Scientific™ GeneJET Genomic DNA Purification Kit
Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
| Column Type | Spin Column |
|---|---|
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |