Enzymes and Inhibitors
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Thermo Scientific™ Buffer Set for Restriction Enzymes
Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.
Thermo Scientific™ TheraPure™ Restriction Enzymes
Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.
| Cut Site | CTCTTC(1/4)^ |
|---|---|
| Content And Storage | Store at -20°C. |
| Form | Liquid |
| Enzyme | Eam1104I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Quantity | 3000 U, 150 μL |
| Type IIS RE | Yes |
| Incubator Temperature | 37°C |
| Concentration | 20 U/μL |
| For Use With (Application) | In vitro Transcription |
| Inactivation Temperature | 65°C |
| Sensitive to Heat Inactivation | Yes |
| Product Type | Restriction Enzyme |
| Research Category | Drug Development |
| Compatible Buffer | 10X Tango Buffer |
| Optimal Reaction Temperature | 37°C |
| Product Line | TheraPure™ |
Thermo Scientific™ Dilution Buffer for Restriction Enzymes
Optimize applications that require diluted enzymes with this dilution buffer.
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
| Polymerase | DNA Polymerase I |
|---|---|
| Product Type | Klenow Fragment |
| Compatible Buffer | Restriction Enzyme, PCR, RT Buffer |
| Concentration | 5 U/μL |
Thermo Scientific™ HincII (HindII) (10 U/μL)
The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | HincII (HindII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ DraI (10 U/μL)
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.
Thermo Scientific™ NheI (10 U/μL)
The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ Eco81I (Bsu36I) (10 U/μL)
The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco81I (Bsu36I) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ PscI (PciI) (10 U/μL)
The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PscI (PciI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ PfeI (TfiI) (10 U/μL)
Cut at G^AWTC sites with PfeI (TfiI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: TfiI).
Thermo Scientific™ XagI (EcoNI) (10 U/μL)
Cut at CCTNN^NNNAGG sites with XagI (EcoNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BstENI, EcoNI).
Thermo Scientific™ Kpn2I (BspEI) (10 U/μL)
The Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer (Isoschizomers: AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Kpn2I (BspEI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 55°C |
| Concentration | 10 U/μL |
Thermo Scientific™ MssI (PmeI) (5 U/μL)
The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | MssI (PmeI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer B |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ SgsI (AscI) (10 U/μL)
The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SgsI (AscI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ XmaJI (AvrII) (10 U/μL)
Cut at C^CTAGG sites with XmaJI (AvrII) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: AspA2I, AvrII, BlnI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | XmaJI (AvrII) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |