HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	170 m²/g
USP Type	L1

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Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

Pricing and Availability
Specifications

Carbon Load	11%
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	170 m²/g
USP Type	L1

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

Pricing and Availability

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Thermo Scientific™ Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Columns

Retain and resolve polar analytes with highly aqueous mobile phases.

Pricing and Availability
Specifications

Pore Size	175 Å
Carbon Load	12%
Temperature	60°C
Phase	Reversed Phase
Endcapped	Polar
pH	2 to 9
Surface Area	220 m²/g
USP Type	L1

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Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns

Use as a robust, general-purpose column for QC/QA applications. They are high-quality, base-deactivated, fully endcapped, have similar C18 selectivity but slightly less retentive.

Pricing and Availability
Specifications

Pore Size	130 Å
Carbon Load	7%
Stationary Phase	C8
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	170 m²/g
USP Type	L7

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Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

Pricing and Availability
Specifications

Pore Size	100 Å
Stationary Phase	C18
Phase	Reversed Phase
USP Type	L1

Thermo Scientific™ Syncronis™ C18 Columns

Use for hydrophobic interaction reversed-phase separations.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	16%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 9
Surface Area	320 m²/g
USP Type	L1

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Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC Columns, 1.5μm

Use the 1.5μm solid core particles of these columns with the Vanquish UHPLC system for unique high-efficiency, high-resolution analyses.

Pricing and Availability
Specifications

Pore Size	80 Å
Particle Size	1.5 μm
Carbon Load	6.5%
Stationary Phase	C18
Temperature	70°C
Phase	Reversed Phase
Endcapped	Yes
Diameter (Metric)	2.1 mm
pH	2 to 9
Surface Area	110 m²/g
USP Type	L1

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Thermo Scientific™ Accucore™ Phenyl-Hexyl HPLC Columns

Use this solid core column for fast, high-resolution separations of aromatic and moderately polar compounds.

Pricing and Availability
Specifications

Pore Size	80 Å
Particle Size	2.6 μm
Carbon Load	5%
Temperature	70°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Ultrapure Silica
pH	2 to 8
Surface Area	130 m²/g
Particle Shape	Solid Core
Max. Pressure	14500 psi (1000 bar)
Product Line	Accucore
USP Type	L11

Thermo Scientific™ Acclaim™ VANQUISH™ Polar Advantage II UHPLC Columns

Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run.

Pricing and Availability
Specifications

Pore Size	120 Å
Stationary Phase	Polar Embedded
Temperature	60°C
Phase	Reversed Phase
For Use With	LC/MS and RSLC
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	300 m²/g
Max. Pressure	21756 psi (1500 bar)
Column Format	Analytical Column
Particle Size	2.2 μm
Carbon Load	17%
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric)	2.1 mm
pH	1.5 to 10
Technique	Bio-LC
Product Line	Acclaim
USP Type	L60

Hamilton™ PRP-1 Reversed Phase HPLC Columns, 5um Particle Size

Stable alternative to C8 or C18 Columns

Pricing and Availability
Specifications

Pore Size	100 Å
Stationary Phase	PSDVB (Polystyrene Divinylbenzene)
Temperature	5°C to 85°C
Phase	Reversed Phase
For Use With	Organic Compounds, Pharmaceuticals, Steroids, Nucleic Acids, Vitamins, Herbicides
Column Type	Reversed Phase
Max. Pressure	5000 psi (345 bar)
Column Format	Analytical Column
Particle Size	5 μm
Type	Column
Packing Material	PSDVB (Polystyrene Divinylbenzene)
Mobile Phase Compatibility	pH 1 to 13, 0 to 100% aqueous, organic modifier
pH	1 to 13
Film Thickness	5 μm
USP Type	L21

Thermo Scientific™ Acclaim™ AmG C18 HPLC Columns

Meet today’s demands for aminoglycoside antibiotic analyses, including drug purity characterization & quantification. They have unique tolerance to harsh conditions.

Pricing and Availability
Specifications

Pore Size	120 Å
Particle Size	3 μm
Stationary Phase	C18
Temperature	<80°C
Phase	Reversed Phase
Packing Material	Spherical, High Purity Silica
Column Type	Aminoglycoside Analysis
pH	0.5 to 10
Green Features	FSC
Max. Pressure	8000 psi (550 bar)
Product Line	Acclaim
USP Type	L1

Thermo Scientific™ BioBasic™ AX HPLC Columns

Use Thermo Scientific™ BioBasic™ AX LC Columns for the separation of proteins, peptides, other anionic species and highly polar molecules.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	3%
Stationary Phase	Polyethyleneimine
Temperature	60°C
pH	2 to 8
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)

Thermo Scientific™ BioBasic™ 4 HPLC Columns

Use this 300Å wide pore silica for analysis of larger hydrophobic proteins and peptides that bind too tightly on C8 or C18 columns.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	4%
Stationary Phase	C4
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 9
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)
USP Type	L26

Thermo Scientific™ BioBasic™ SCX HPLC Columns

BioBasic SCX LC Columns are optimized for the separation of proteins, peptides and other cationic species.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	3%
Stationary Phase	Sulfonic Acid
Temperature	60°C
Phase	Ion Exchange
Endcapped	No
Column Type	Ion Exchange
pH	2 to 8
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	BioBasic
USP Type	L52

HPLC Columns

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Thermo Scientific™ Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC Columns, 1.5μm SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Thermo Scientific™ Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Columns

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns

Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC Columns, 1.5μm