Neon NxT Electroporation System

Unlike standard cuvette-based electroporation chambers, the Neon™ NxT electroporation system uses a proprietary biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows for a better maintenance of physiological conditions resulting in high cell survival compared to conventional electroporation.

• Creates a more uniform electric field
• Maintains a stable pH throughout the electroporation chamber
• Generates minimal heat
• Forms fewer ions
• Reduces shear force experienced by cells

The benefits of the Neon™  NxT Electroporation System over other electroporation systems are listed below:

• Small to large number of cells can be used. The transfection is performed using as few as 2 x 10E4 or as many as 10 x 10E6 cells per reaction using a sample volume of 10 µL or 100 µL
• The Neon NxT Electroporation System uses a single transfection kit (Neon NxT Kit) that is compatible with various mammalian cell types, including primary and stem cells, thereby avoiding the need to determine an optimal buffer for each cell type. The two NxT resuspension buffers cover all cell types and applications: Neon NxT Resuspension T Buffer for high voltage protocols >1900 V (naive T cells) and Neon NxT Resuspension R Buffer for all other protocols
• Open and transparent protocols that are optimized for ease of use and simplicity. The Transfection Select Protocols & Citations Library contains optimized protocols for many commonly-used cell types (thermofisher.com/transfectionprotocolsandcitations)
• The Neon NxT Device is preprogrammed with 24-well optimization protocols to optimize conditions for all payloads and cell types
• The Neon NxT Electroporation System leverages Neon technology that produces a more uniform electrical field and a lower pH gradient across the cell suspension. Therefore, this design allows for a better maintenance of physiological conditions resulting in very high cell survival compared to conventional electroporation

Yes. The Neon™ NxT Electroporation System can be used for electroporation of any RNAi substrate (siRNA, shRNA, miRNA). You can use the same conditions described in the cell type-specific protocol for DNA, or use the pre-programmed 24-well optimization protocols included in the Neon NxT Device.