MACHEREY-NAGEL

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A Company with Tradition and Future

MACHEREY-NAGEL was founded in 1911 in Düren as a specialist manufacturer of filter papers. Since that time, we have evolved into an innovative international company for chemical and biomolecular analysis. MACHEREY-NAGEL stands for innovative and creative solutions which we have a penchant for developing in-house. In addition to the “traditional” departments, such as research, production, sales and media design, we also have departments such as mechanical engineering and device development as well as our own joinery.

Our products are among the world’s most reliable analytical systems. They are used, for example, in industry, healthcare, biotechnology, environmental analysis and research. Numerous patents and international certifications underline the high quality of our products and the competence of our employees.

MACHEREY-NAGEL
Nucleic Acid Purification
MN
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Plasmid DNA

As a pioneer in nucleic acid purification technologies, MN has been developing plasmid purification products of the highest quality for about 30 years. Today we offer a comprehensive portfolio of plasmid purification kits enabling the highest purity, excellent yield and very fast processing.

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Clean-up Kits

MACHEREY‑NAGEL’s clean up kits focus on fast, easy to handle, and quantitative high recovery DNA and RNA purification. Based on different purification technologies, you can select the optimal solution for low to high throughput as well as for high quality downstream applications.

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DNA Purification Kits

Combining selective purification technologies and chemistries, we offer a comprehensive range of specialised DNA isolation kits for a broad range of diversified starting materials. The resulting high quality DNA serves as an excellent basis for following demanding downstream applications.

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RNA Purification Kits

Our ready to use kits isolate high quality RNA including small RNA (miRNA) from various sample material with highest lysis efficiency. The handling is convenient and user‑friendly. Take advantage of our experience as RNA experts.

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Viral RNA/DNA

Our viral RNA and DNA isolation kits have been optimised for use with cell‑free biological fluids and diverse veterinary and clinical samples. They are specialised for low viral titers and large sample volumes and are characterised by the highest consistency and sensitivity for different types of viruses.

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Protein

We provide ready to use resins or columns for GST‑tag and His‑tag purification. Our products show market leading performance. Using our protein purification products you can save money without making compromises.

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Rapid Tests
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pH Tests

A large variety of different pH test paper types is provided by MN:

  • pH Fix – Non bleeding pH test strips
  • pH universal indicator paper – Standard in many applications
  • PEHANON – pH determination in coloured samples

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Semi Quantitative Test Strips

Test papers and test strips for semi quantitative analysis are extremely simple to handle. They are based on the principle of “Dip&Read”. They are available for a variety of parameters and comply with all requirements of a modern rapid test. The evaluation of these tests is carried out by a comparison with a colour scale.

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Qualitative Test Papers – Without Colour Chart

Qualitative test papers come without a colour scale. With little effort, the presence of ions and other substances can be detected. The test papers change their colour when the concentration is above the specified limit of detection.

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Featured Products

Filtration
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Cellulose Filters

  • Ashless filter papers
  • Ashless hardened filter papers
  • Qualitative filter papers
  • Qualitative hardened filter papers
  • Technical filter papers
  • Chromatography papers

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Glass Fibre Filters

  • Glass fibre filters without binder
  • Glass fibre filters with binder
  • Quartz fibre filters
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Extraction Thimbles

  • Cellulose extraction thimbles
  • Glass fibre extraction thimbles

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Membranes

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Special Filter Products

  • Weighing aids
  • Phase separation papers
  • Lens paper - José papers
  • Ion exchange papers
  • Antibiotic resistency papers
  • Surface protection papers
  • Filtering aids
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Syringe Filters

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Featured Products

TLC Products

MACHEREY-NAGEL supply laboratories around the world with HPLC, GC and SPE columns, TLC/HPTLC plates, autosampler vials, closures and more.

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MACHEREY-NAGEL Chromatography – Complete Solutions for your Analysis

We provide optimal and reliable solutions for your laboratory work in sample preparation, method development and routine analysis.

From a pioneer in chromatography to a full-range supplier of laboratory consumables, we supply laboratories around the world with HPLC, GC and SPE columns, TLC/HPTLC plates, autosampler vials, closures and more.

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MACHEREY-NAGEL offers a versatile range of ready-to-use TLC and HPTLC layers, which are the result of 50 years of continuous research and development in thin layer chromatography.


The Comprehensive Range of TLC Products from MACHEREY-NAGEL Includes:

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TLC/HPTLC glass plates

ADAMANT, SIL G or SIL HD are silica layers on glass backing. It’s hard to imagine a laboratory without our reliable TLC and HPTLC glass plates.

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ALUGRAM and POLYGRAM TLC/HPTLC sheets are easy to handle, lightweight and flexible.

High performance silica on ALUGRAM Xtra sheets provides outstanding wettability for precise colourisation results. Moreover, these TLC sheets are easy to cut with scissors.

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Accessories

In addition to high quality TLC plates and sheets, we offer staining reagents and developing chambers, as well as introductory sets for educational purposes.

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TLC Backings & Supports

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TLC/HPTLC plates and sheets consist of an adsorbent stationary phase on a backing support.

MACHEREY-NAGEL offers three different backings:
 

  • Glass plates
  • Aluminium sheets
  • Polyester sheets

Properties of TLC backings

Glass Plates

Glass plates are robust, heat proof and chemically resistant to all common mobile phases and visualisation reagents. They are the traditional option in TLC/HPTLC and are widely used in the pharmaceutical sector and other laboratory environments.

  • Thickness (approx.): 1.3mm
  • Weight and storage requirements: high
  • Torsional strength: ideal
  • Temperature stability: high
  • Susceptible to breakage: yes
  • Can be cut with scissors: no
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: high
  • Suitability for aqueous detection reagents: depends on phases (layer)
 

Aluminium Sheets - ALUGRAM Xtra

Aluminium sheets are easy to handle, lightweight and flexible. High performance silica on ALUGRAM Xtra sheets provides outstanding wettability for precise colourisation results, even with 100% aqueous detection reagents. Moreover, ALUGRAM Xtra sheets are easy to cut with scissors. No flaking of silica occurs!

  • Thickness (approx.): 0.15mm
  • Weight and storage requirements: low
  • Torsional strength: relatively high
  • Temperature stability: high
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: low
  • Suitability for aqueous detection reagents: ALUGRAM: low to moderate, ALUGRAM Xtra: moderate to high
 

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Polyester Sheets – POLYGRAM

Polyester sheets are easy to handle, lightweight and flexible, and can also be cut with scissors. Developed POLYGRAM sheets can also be stored for documentation in laboratory notebooks.

  • Thickness (approx.): 0.2mm
  • Weight and storage requirements: low
  • Torsional strength: low
  • Temperature stability: maximum 185°C
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: high
  • Suitability for aqueous detection reagents: good

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TLC and HPTLC

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Features of modern TLC/HPTLC

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:

  • High sample throughput in a short time
  • Suitable for screening tests
  • Pilot procedure for HPLC and Flash chromatography
  • After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)
  • Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date
  • Rapid and cost-efficient optimisation of the separation due to easy change of mobile and stationary phase
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'TLC' and 'HPTLC' are both widely used terms in laboratory environments, but what is the difference? At MACHEREY-NAGEL, we define 'HPTLC' where at least one of the following criteria is being fulfilled:

  • Layers with particle size 2 to 10μm
  • Layers prefixed with 'Nano'
  • Modified layers

Narrow fractionation of HPTLC silica particles allows:

  • Sharper separations
  • Shorter developing times
  • Shorter migration distances
  • Smaller samples needed
  • Increased detection sensitivity (compared to TLC)

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