Thermo Scientific™ Y-PER™ Yeast Protein Extraction Reagent

Beschreibung

Thermo Scientific™ Y-PER Yeast Protein Extraction Reagent is the first commercially available yeast lysis reagent to use a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.

This detergent-based cell lysis buffer eliminates the need to use glass beads or mechanical disruption to break through the thick proteinaceous cell envelope to extract protein Y-PER Reagent is effective for S. cerevisiae and other popular species, making it applicable for use in fusion-tagged protein purification and reporter enzyme assays with these model organisms. The lysis reagent also can be used for genomic and plasmid DNA extraction from yeast. The standard protocol for protein extraction is easy: simply add an appropriate volume of Y-PER Reagent to pelleted yeast cells, incubate at room temperature for approximately 20 minutes and spin down the debris. The resulting supernatant is a concentrated protein solution, surpassing typical yields obtained with traditional glass bead disruption.

Highlights:

• More protein – extracts more than twice as much protein as glass bead methods

• Less hassle – eliminates the difficulties associated with traditional glass bead lysis (e.g., clinging static-charged beads, protein/bead clumps and runaway beads)

• Optmized for yeast – validated for use with Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris

• Versatile – effective for many different organisms, including Bacillus subtilus and both Gram-positive and Gram-negative bacteria; suitable for use in a diverse range of situations