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IBA Solutions For Life Science StarGate™ pESG-IBA Acceptor Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

Brand:  IBA Solutions For Life Science 5-4505-001

Additional Details : Weight : 0.00010kg

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Product Code. 15623798

  • 213.00€ / 5µg


Estimated Shipment date
from Supplier 24-07-2019
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Description

Description

pESG-IGA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori. NeoR for generation of stable cells lines.

  • Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
  • SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
  • ColE1 + Amp: for propagation in E. coli
  • NeoR: neomycin resistance gene to generate stable cell lines
  • BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

StarGate™ System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

  • Minimal extra modification of the gene due to short combinatorial sites
  • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
  • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
  • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
  • The final expression vector, the Destination Vector, is then placed into the respective host
Specifications

Specifications

250 ng/μL
Liquid
pESG-IBA 105
Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
8
Acceptor Vector for mammalian expression
2-8°C for frequent usage, -20°C for long-term storage
Twin-Strep-tag™ for N-terminal 
Clear
StarGate™ system, mammalian host cells
SV40 large T antigen (e.g. COS1 or COS7)
Neomycin resistance gene
Human cytomegalovirus (CMV) immediate-early promoter
5 μg
20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μg
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