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  6. 11964852

OPTIZYME™ BglII, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8014-1

Additional Details : Weight : 0.01000kg

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Product Code. 11964852

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance
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Description

Description

5'...A^G A T C T...3'
3'...T C T A G^A...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 50 - 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 8.2 at 25°C), 200 mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with BglII.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of BglII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: BamHI, BclI, Bsp143I, MboI, PsuI
  • Methylation Effects:
  • Dam: Completely overlaps - no effect
    • AGm6ATCT
  • (No effect on cleavage)
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - cleavage impaired
    • 5'...TGm6AGATCT(N)3 TGCT...3'
  • 3'...AC TCTAGA(N)3m6ACGA...5'
  • (Cleavage is impaired)

    • Specifications

    Specifications

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
    Keep container tightly closed
    A.GATCT
    Liquid
    25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
    7.4
    10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
    1000 U
    BglII
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