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Proteintech™ Human NGAL ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous NGAL
Brand: Proteintech™ KE00144-96T
Additional Details : Weight : 0.15000kg
Description
- Recognizes human NGAL
- NGAL (Neutrophil gelatinase-associated lipocalin, Lipocalin-2, LCN2) is a secreted glycoprotein that belongs to lipocalin protein family
- NGAL exists as a monomer, homodimer, disulfide-linked heterodimer in cells, covalently conjugated with gelatinase in order to decrease degradation
- Roles include iron trafficking, chemotactic, promote cell differentiation and proliferation
- Found in human neutrophils and expressed in tissues containing renal, uterus, liver and spleen, cardiomyocytes, neurons, immune cells
- NGAL is a biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation, metabolic diseases
- Detected in human serum, saliva, urine
Process:
- Antibody specific for NGAL pre-coated onto microwells
- NGAL protein in samples captured by the coated antibody after incubation
- After extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for NGAL is added to detect captured NGAL protein
- For signal development, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
| NM_005564 | |
| 3.3 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 3.5 hr. |
| 78.1 to 5000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 3934 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P80188 |